Laboratory for Animal Nutrition and Animal Product Quality, Department of Animal Production, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium.
Br J Nutr. 2010 Jul;104(2):204-13. doi: 10.1017/S0007114510000541. Epub 2010 Mar 4.
The present study was conducted to evaluate the effect of different dietary lipid sources, age and sex on the SFA and MUFA metabolism in broiler chickens using a whole body fatty acid balance method. Four dietary lipid sources (palm fat, Palm; soyabean oil, Soya; linseed oil, Lin; and fish oil, Fish) were added at 3 % to a basal diet containing 5 % Palm. Diets were fed to female and male chickens from day 1 to either day 21 or day 42 of age. The accumulation (percentage of net intake and ex novo production) of SFA and MUFA was significantly lower in broilers fed on Palm than in broilers fed on the other diets (85.7 v. 97.4 %). Conversely, beta-oxidation was significantly higher in Palm-fed birds than the average of the other dietary treatments (14.3 v. 2.6 %). On average, 33.1 % of total SFA and MUFA accumulated in the body were elongated, and 13.8 % were Delta-9 desaturated to longer chain or more unsaturated metabolites, with lower proportions being elongated and desaturated for the Palm and Fish diets than for the Soya and Lin diets. Total in vivo apparent elongase activity decreased exponentially in relation to the net intake of SFA and MUFA, while it increased with age. Total in vivo apparent Delta-9 desaturase activity was not significantly affected by dietary treatment or age. Total ex novo production and beta-oxidation of SFA and MUFA showed a negative and positive curvilinear relationship with net intake of SFA and MUFA, respectively. Sex had no effect on SFA and MUFA metabolism.
本研究采用全身体脂肪酸平衡法，评估了不同饲粮脂质来源、日龄和性别对肉鸡饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）代谢的影响。在含有 5%棕榈油的基础饲粮中分别添加 3%的 4 种饲粮脂质来源（棕榈油、大豆油、亚麻籽油和鱼油），饲粮分别喂给 121 日龄或 142 日龄的母鸡和公鸡。棕榈油组肉鸡 SFA 和 MUFA 的蓄积量（净摄入量和从头合成的百分比）显著低于其他组（85.7%比 97.4%）。相反，棕榈油组肉鸡的β-氧化显著高于其他饲粮处理组的平均值（14.3%比 2.6%）。平均而言，33.1%的总 SFA 和 MUFA 在体内被延长，13.8%被Δ9 去饱和为更长链或更不饱和的代谢物，棕榈油和鱼油饲粮的延长和去饱和比例低于大豆油和亚麻籽油饲粮。体内总表观延长酶活性与 SFA 和 MUFA 的净摄入量呈指数关系下降，而随日龄增加而增加。体内总表观Δ9 去饱和酶活性不受饲粮处理或日龄的影响。SFA 和 MUFA 的总从头合成和β-氧化与 SFA 和 MUFA 的净摄入量呈负和正曲线关系。性别对 SFA 和 MUFA 代谢没有影响。
