上海养鸡场沙门氏菌的血清型、基因型和抗菌药物敏感性分析。

Serotype, genotype, and antimicrobial susceptibility profiles of Salmonella from chicken farms in Shanghai.

机构信息

Department of Food Science and Bor Luh Food Safety Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

出版信息

J Food Prot. 2010 Mar;73(3):562-7. doi: 10.4315/0362-028x-73.3.562.

DOI:10.4315/0362-028x-73.3.562

PMID:20202346

Abstract

Five hundred fifty samples were collected from five chicken farms in Shanghai during March 2005 to October 2006. Twenty-five samples tested positive for Salmonella from a total of 550 samples, of which 500 were obtained from feces of healthy chickens and 50 were obtained from diseased chicks. The 25 presumptive Salmonella isolates were confirmed by the API 20E identification kit. Serotyping of these isolates by agglutination tests with antiserum displayed seven serovars; genotyping of these isolates with multilocus sequence typing demonstrated six sequence type (ST) patterns (i.e., ST-11, ST-19, ST-92, ST-96, ST-290, and ST-367). The multilocus sequence typing data revealed that some of these strains, isolated from different farms, might have the same ST and might come from the same source. The susceptibilities of these strains to 14 antimicrobials were determined; most of the isolates (13 of 25) were resistant to doxycycline and tetracycline, and two isolates were resistant to cefotaxime and ceftazidime, but none was resistant to gentamicin or kanamycin.

摘要

从 2005 年 3 月到 2006 年 10 月，在上海的 5 个鸡场采集了 550 个样本。在总共 550 个样本中，有 25 个样本检测出沙门氏菌呈阳性，其中 500 个来自健康鸡的粪便，50 个来自患病雏鸡。这 25 个疑似沙门氏菌分离株通过 API 20E 鉴定试剂盒得到确认。通过与抗血清的凝集试验对这些分离株进行血清型鉴定显示有 7 个血清型；通过多位点序列分型对这些分离株进行基因分型显示有 6 种序列型（即 ST-11、ST-19、ST-92、ST-96、ST-290 和 ST-367）。多位点序列分型数据显示，从不同农场分离出的一些菌株可能具有相同的 ST，可能来自同一来源。这些菌株对 14 种抗菌药物的敏感性进行了测定；大多数分离株（25 株中的 13 株）对强力霉素和四环素具有耐药性，有 2 株对头孢噻肟和头孢他啶具有耐药性，但对庆大霉素或卡那霉素均无耐药性。