Universidade Estadual de Campinas (UNICAMP), Departamento de Genética, Evolução e Bioagentes do Instituto de Biologia, SP 13083-970, Brazil.
Micron. 2010 Jul;41(5):484-9. doi: 10.1016/j.micron.2010.01.011. Epub 2010 Feb 11.
The molecular and ultrastructural profiles of the symbionts found in the midgut and ileum of Cephalotes atratus, Cephalotes clypeatus, and Cephalotes pusillus were determined using the V3 region of the bacterial 16S rDNA gene and transmission electron microscopy (T.E.M.). Two samples of C. atratus, three of C. clypeatus, and six of C. pusillus were analyzed. The coefficients of similarity ranged from 80% to 94% for the samples of symbionts from C. clypeatus and C. atratus, despite being collected in geographically distant sites. The variability within symbionts found in the samples of C. pusillus varied from 29% to 55%, in samples geographically close as well as distant. PCR-DGGE was effective for the purpose of this study and can be considered a versatile tool to analyze gut microbiota. Details of the ultrastructural aspect of these bacteria are presented.
采用细菌 16S rDNA 基因 V3 区和透射电子显微镜（TEM）的方法，对 Cephalotes atratus、Cephalotes clypeatus 和 Cephalotes pusillus 中中肠和回肠共生体的分子和超微结构特征进行了研究。分析了 2 个 Cephalotes atratus 样本、3 个 Cephalotes clypeatus 样本和 6 个 Cephalotes pusillus 样本。尽管采集地点相距甚远，但 Cephalotes clypeatus 和 Cephalotes atratus 共生体样本的相似系数范围为 80%至 94%。 Cephalotes pusillus 样本中发现的共生体的变异性在地理上接近和遥远的样本中从 29%到 55%不等。PCR-DGGE 对本研究是有效的，可以被认为是分析肠道微生物组的多功能工具。本文还介绍了这些细菌的超微结构细节。
