Nonclercq D, Toubeau G, Lambricht P, Heuson-Stiennon J A, Laurent G
Laboratory of Histology and Experimental Cytology, Faculty of Medicine, University of Mons, Belgium.
Nephron. 1991;57(2):210-5. doi: 10.1159/000186253.
Tubular necrosis elicits a process of renal tissue repair characterized by an increase of cell turnover in tubular epithelium. The present study was undertaken to examine the distribution of epidermal growth factor (EGF) and/or of its larger precursor proEGF in the kidney undergoing tubular regeneration. Sprague-Dawley rats were exposed to various drugs (aminoglycosides or platinum-based anticancer agents) known to induce tubular necrosis. The proliferative response resulting from renal tissue damage was measured by the incorporation of [3H]thymidine into DNA of renal cells. EGF immunoreactivity was evidenced by immunocytochemical staining, using anti-EGF antibody and immunogold-silver staining. Concomitantly with the increase of cell proliferation resulting from tubular injury, a redistribution of EGF immunoreactivity was observed in renal tissue (from the inner stripe of outer medulla towards renal cortex). Amazingly, EGF was detected in proximal tubules of nephrotoxin-treated rats whereas, in the kidneys of control animals, it was almost exclusively found in distal tubules and collecting ducts. Insofar as the administration of exogenous EGF has recently been shown to enhance renal tubular regeneration after ischaemic injury [Humes et al: J Clin Invest 1989; 84:1757-1761], our observations lend further support to the concept that EGF might be involved in renal tissue repair.
肾小管坏死引发肾组织修复过程，其特征为肾小管上皮细胞更新增加。本研究旨在检测表皮生长因子（EGF）及其较大前体proEGF在经历肾小管再生的肾脏中的分布。将Sprague-Dawley大鼠暴露于已知可诱导肾小管坏死的各种药物（氨基糖苷类或铂类抗癌剂）。通过将[3H]胸腺嘧啶掺入肾细胞DNA中来测量肾组织损伤引起的增殖反应。使用抗EGF抗体和免疫金银染色，通过免疫细胞化学染色证明EGF免疫反应性。伴随着肾小管损伤导致的细胞增殖增加，在肾组织中观察到EGF免疫反应性的重新分布（从外髓质内带到肾皮质）。令人惊讶的是，在肾毒素处理大鼠的近端小管中检测到EGF，而在对照动物的肾脏中，它几乎仅存在于远端小管和集合管中。鉴于最近已证明给予外源性EGF可增强缺血性损伤后的肾小管再生[Humes等人：《临床研究杂志》1989年；84:1757 - 1761]，我们的观察结果进一步支持了EGF可能参与肾组织修复的概念。
