Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology, Am Fasanengarten, 76131 Karlsruhe, Germany.
Bioresour Technol. 2010 Jul;101(14):5159-67. doi: 10.1016/j.biortech.2010.02.015. Epub 2010 Mar 4.
In this study, a denitrifying culture was enriched in a continuously re-circulated anoxic suspension reactor fed with glucose and nitrate for about 8 months (stage I) under different organic loading rates (OLR). At the end of stage I, the removal efficiency for NO(3)(-)-N was 80% with 93% COD (5 g/l) removal at an OLR of 2.5 g/ld. The mean COD/N removal ratio during the whole enrichment was 3.3. The response to phenol as a toxic substance on glucose enriched culture for long time period was investigated by introducing phenol as a co-substrate in the reactor feed in stage II. Phenol was increased gradually to 753 mg/l till termination of the reactor operation. After increasing the OLR or the phenol concentration, fluctuations in removal efficiencies were observed which were partly reversible. At the end of the reactor operation, NO(3)(-)-N removal was 65% with 81% COD removal at a phenol degradation rate of 207 mg/ld phenol. The OLR of the reactor was 4.3g/ld COD and a hydraulic retention time (HRT) of 1 day. Phenol degradation in batch assays under anoxic conditions and at low phenol concentrations (188 mg/l) proceeded a removal rate of 1.2g/l which decreased to 0.67 mg/ld at high phenol concentration (847 mg/l).
在本研究中，采用连续再循环缺氧悬浮反应器，在不同有机负荷率（OLR）下，用葡萄糖和硝酸盐约 8 个月（第一阶段）富集反硝化培养物。在第一阶段结束时，在 OLR 为 2.5 g/ld 时，NO3--N 的去除效率为 80%，COD（5 g/l）去除率为 93%。整个富集过程中平均 COD/N 去除比为 3.3。通过在第二阶段将苯酚作为有毒物质引入反应器进料中，研究了长期用葡萄糖富集培养物对苯酚的反应。逐渐将苯酚增加到 753 mg/l，直到反应器停止运行。当增加 OLR 或苯酚浓度时，去除效率会发生波动，部分是可逆的。在反应器运行结束时，在苯酚降解速率为 207 mg/ld 苯酚时，NO3--N 的去除率为 65%，COD 去除率为 81%。反应器的 OLR 为 4.3g/ld COD，水力停留时间（HRT）为 1 天。在缺氧条件和低苯酚浓度（188 mg/l）下进行的批处理试验中，苯酚的去除率为 1.2g/l，在高苯酚浓度（847 mg/l）下降低至 0.67 mg/ld。
