Department of Ophthalmology, China Medical University Hospital, Taichung, Taiwan.
Invest Ophthalmol Vis Sci. 2010 Jul;51(7):3462-7. doi: 10.1167/iovs.09-4921. Epub 2010 Mar 5.
PURPOSE. The migration and invasion of tumor cells correlate with the interaction between MMP and TIMP. Therefore, the purpose of this study was to determine the role of MMP-9, MMP-10, and TIMPs in pterygium formation and progression. METHODS. MMP-9, MMP-10, and TIMP proteins were studied using immunohistochemistry on 82 pterygial specimens and 30 normal conjunctivas. Pterygium epithelial cells (PECs), cultured in a serum-free culture medium, and siRNA were used to knock down TIMP gene expression to understand the role of TIMP in pterygium invasion. RESULTS. Among the 82 pterygial samples, 29 specimens (35.4%) were positive for MMP-9 expression, 28 were positive for MMP-10 (34.1%), and 59 were positive for TIMP1 (72.0%). Staining for MMPs was limited to the cytoplasm of the epithelial layer. The TIMP staining was detected in the pterygium epithelium, fibroblasts and corneal epithelium. In the cell model, cell invasion and migration ability increased in TIMP knockdown PECs compared with the parental control. CONCLUSIONS. MMP-9 and MMP-10 may each play a role in pterygium formation, and TIMPs may contribute to pterygium invasion inhibition.
目的。肿瘤细胞的迁移和浸润与 MMP 和 TIMP 的相互作用有关。因此，本研究旨在确定 MMP-9、MMP-10 和 TIMPs 在翼状胬肉形成和进展中的作用。
方法。我们使用免疫组织化学方法研究了 82 例翼状胬肉标本和 30 例正常结膜中的 MMP-9、MMP-10 和 TIMP 蛋白。在无血清培养培养基中培养翼状胬肉上皮细胞（PECs），并使用 siRNA 敲低 TIMP 基因表达，以了解 TIMP 在翼状胬肉侵袭中的作用。
结果。在 82 例翼状胬肉样本中，29 例（35.4%）MMP-9 表达阳性，28 例（34.1%）MMP-10 表达阳性，59 例（72.0%）TIMP1 表达阳性。MMPs 的染色仅限于上皮层的细胞质。TIMP 的染色在翼状胬肉上皮细胞、成纤维细胞和角膜上皮细胞中均有检测到。在细胞模型中，与亲本对照相比，TIMP 敲低 PECs 的细胞侵袭和迁移能力增加。
结论。MMP-9 和 MMP-10 可能各自在翼状胬肉形成中发挥作用，而 TIMPs 可能有助于抑制翼状胬肉的侵袭。
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