重新审视戊二醛交联：以精氨酸-赖氨酸分子间双联体为例。
Revisiting glutaraldehyde cross-linking: the case of the Arg-Lys intermolecular doublet.
作者信息
Salem Michèle, Mauguen Yves, Prangé Thierry
机构信息
Laboratoire de Cristallographie et RMN Biologiques (UMR 8015 CNRS), Faculté de Pharmacie, Université Descartes, 4 Avenue de l'Observatoire, 75006 Paris, France.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):225-8. doi: 10.1107/S1744309109054037. Epub 2010 Feb 23.
Abstract
In addition to the common use of glutaraldehyde to nonspecifically cross-link protein crystals through lysine residues disposed on the surface of the protein, the use of gentle vapour diffusion of glutaraldehyde offers a convenient way to limit polymerization and to allow slow diffusion throughout the crystal. In the case of trimeric barnase crystals, a specific cross-link was observed between an lysine side chain and an arginine side chain that were spatially disposed at the ideal distance on the protein surface in the three monomers. Here, the direct observation of a specific Lys-Arg cross-link site is reported and a mechanism is proposed for the reaction.
摘要
除了常用戊二醛通过蛋白质表面的赖氨酸残基进行非特异性交联蛋白质晶体外，采用温和的戊二醛蒸汽扩散法提供了一种限制聚合反应并使戊二醛在整个晶体中缓慢扩散的便捷方法。在三聚体核糖核酸酶晶体的情况下，观察到一个赖氨酸侧链和一个精氨酸侧链之间存在特异性交联，这两个侧链在三个单体的蛋白质表面上以理想距离空间排列。本文报道了对一个特定赖氨酸-精氨酸交联位点的直接观察结果，并提出了该反应的机制。
相似文献
1
Revisiting glutaraldehyde cross-linking: the case of the Arg-Lys intermolecular doublet.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):225-8. doi: 10.1107/S1744309109054037. Epub 2010 Feb 23.
2
Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X-ray structure analysis.
Biotechnol Bioeng. 2007 Oct 15;98(3):711-8. doi: 10.1002/bit.21459.
3
On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations.
Biochim Biophys Acta. 2006 May;1764(5):903-12. doi: 10.1016/j.bbapap.2006.02.009. Epub 2006 Mar 20.
4
Partial acetylation of lysine residues improves intraprotein cross-linking.
Anal Chem. 2008 Feb 15;80(4):951-60. doi: 10.1021/ac701636w. Epub 2008 Jan 18.
5
Alternative reaction mechanism for the cross-linking of gelatin with glutaraldehyde.
J Agric Food Chem. 2010 Jan 27;58(2):998-1003. doi: 10.1021/jf9031603.
6
Glutaraldehyde-mediated protein immobilization.
Methods Mol Biol. 2013;1051:33-41. doi: 10.1007/978-1-62703-550-7_3.
7
X-ray crystallographic studies of the denaturation of ribonuclease S.
Proteins. 1999 Aug 15;36(3):282-94.
8
Thermostabilization of Bacillus amyloliquefaciens alpha-amylase by chemical cross-linking.
J Biotechnol. 2006 Jun 10;123(4):434-42. doi: 10.1016/j.jbiotec.2005.12.017. Epub 2006 Jan 30.
9
Chemical reactions in cottonseed protein cross-linking by formaldehyde, glutaraldehyde, and glyoxal for the formation of protein films with enhanced mechanical properties.
J Agric Food Chem. 2001 Oct;49(10):4676-81. doi: 10.1021/jf0101152.
10
A novel dynamic heterogeneous phase polymerization reaction for poly-hemoglobin with narrow molecular weight distribution.
Artif Cells Blood Substit Immobil Biotechnol. 2008;36(5):439-44. doi: 10.1080/10731190802375786.
引用本文的文献
1
The structure of the Tad pilus alignment complex reveals a periplasmic conduit for pilus extension.
Nat Commun. 2025 Jul 29;16(1):6977. doi: 10.1038/s41467-025-62457-8.
2
Characterization of DnaB-DnaG Interaction in M. tuberculosis Using Small-Angle X-ray Scattering-Based Dissociation Assay.
Chembiochem. 2025 Jul 18;26(14):e202500289. doi: 10.1002/cbic.202500289. Epub 2025 Jul 1.
3
Vegetative and microbial proteins for bioplastics applications - a review in the indian context.
RSC Adv. 2025 May 20;15(21):16392-16432. doi: 10.1039/d4ra08544b. eCollection 2025 May 15.
4
Altering the Properties of Laccases from () and by Chemical Modifications of Proteins.
Biomolecules. 2025 Apr 4;15(4):531. doi: 10.3390/biom15040531.
5
Collagen crosslinking-induced corneal morphological changes: a three-dimensional light sheet Microscopy-based evaluation.
Sci Rep. 2024 Nov 16;14(1):28330. doi: 10.1038/s41598-024-78516-x.
6
pH-Responsive Collagen Hydrogels Prepared by UV Irradiation in the Presence of Riboflavin.
Int J Mol Sci. 2024 Sep 27;25(19):10439. doi: 10.3390/ijms251910439.
7
A digital microfluidic device integrated with electrochemical sensor and 3D matrix for detecting soluble PD-L1.
Biosens Bioelectron X. 2024 Aug;19. doi: 10.1016/j.biosx.2024.100490. Epub 2024 May 17.
8
Stoichiometry and architecture of the human pyruvate dehydrogenase complex.
Sci Adv. 2024 Jul 19;10(29):eadn4582. doi: 10.1126/sciadv.adn4582. Epub 2024 Jul 17.
9
Evidence of Gas Phase Glucosyl Transfer and Glycation in the CID/HCD-Spectra of S-Glucosylated Peptides.
Int J Mol Sci. 2024 Jul 8;25(13):7483. doi: 10.3390/ijms25137483.
10
Influence of Surface Treatments on Urea Detection Using Si Electrolyte-Gated Transistors with Different Gate Electrodes.
Micromachines (Basel). 2024 May 5;15(5):621. doi: 10.3390/mi15050621.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
A short history of SHELX.
Acta Crystallogr A. 2008 Jan;64(Pt 1):112-22. doi: 10.1107/S0108767307043930. Epub 2007 Dec 21.
3
Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction by X-ray structure analysis.
Biotechnol Bioeng. 2007 Oct 15;98(3):711-8. doi: 10.1002/bit.21459.
4
On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations.
Biochim Biophys Acta. 2006 May;1764(5):903-12. doi: 10.1016/j.bbapap.2006.02.009. Epub 2006 Mar 20.
5
Post-crystallization treatments for improving diffraction quality of protein crystals.
Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1173-80. doi: 10.1107/S0907444905019451. Epub 2005 Aug 16.
6
Coot: model-building tools for molecular graphics.
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. doi: 10.1107/S0907444904019158. Epub 2004 Nov 26.
7
INTERMOLECULAR CROSS LINKING OF A PROTEIN IN THE CRYSTALLINE STATE: CARBOXYPEPTIDASE-A.
Proc Natl Acad Sci U S A. 1964 Sep;52(3):833-9. doi: 10.1073/pnas.52.3.833.
8
Refinement and structural analysis of barnase at 1.5 A resolution.
Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):386-98. doi: 10.1107/s0907444998010865.
9
Viscoelastic properties of protein crystals: triclinic crystals of hen egg white lysozyme in different conditions.
Biopolymers. 1981 Mar;20(3):451-67. doi: 10.1002/bip.1981.360200304.
10
Glutaraldehyde as a protein cross-linkage reagent.
J Mol Biol. 1968 Oct 14;37(1):231-3. doi: 10.1016/0022-2836(68)90086-7.
Zotero 插件