表型高通量筛选技术在心血管研究中的应用。
The application of phenotypic high-throughput screening techniques to cardiovascular research.
机构信息
Center for Cardiovascular Research, Washington University School of Medicine, MO 63110, USA.
出版信息
Trends Cardiovasc Med. 2009 Aug;19(6):207-12. doi: 10.1016/j.tcm.2009.12.006.
Abstract
In traditional pure protein high-throughput drug screens, also called in vitro screens, individual compounds from a small molecule collection are tested to determine whether they inhibit the enzymatic activity or binding properties of a purified target protein. In contrast, phenotypic high-throughput drug screens, also called chemical genetic or in vivo screens, investigate the ability of individual compounds from a collection to inhibit a biological process or disease model in live cells or intact organisms. In this review, the role of phenotypic screening techniques to identify novel therapeutic agents for the treatment of cardiovascular disease will be discussed.
摘要
在传统的纯蛋白高通量药物筛选（也称为体外筛选）中，从小分子化合物库中测试单个化合物，以确定它们是否抑制纯化靶蛋白的酶活性或结合特性。相比之下，表型高通量药物筛选（也称为化学遗传学或体内筛选）研究化合物库中单个化合物抑制活细胞或完整生物体中生物过程或疾病模型的能力。在这篇综述中，将讨论表型筛选技术在鉴定治疗心血管疾病的新型治疗剂中的作用。
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