Corbeil Denis, Marzesco Anne-Marie, Fargeas Christine A, Huttner Wieland B
Tissue Engineering Laboratories, BIOTEC, Technische Universität Dresden, Tatzberg 47-49, 01307, Dresden, Germany.
Subcell Biochem. 2010;51:399-423. doi: 10.1007/978-90-481-8622-8_14.
The apical plasma membrane of polarized epithelial cells is composed of distinct subdomains, that is, planar regions and protrusions (microvilli, primary cilium), each of which are constructed from specific membrane microdomains. Assemblies containing the pentaspan glycoprotein prominin-1 and certain membrane lipids, notably cholesterol, are characteristic features of these microdomains in apical membrane protrusions. Here we highlight the recent findings concerning the molecular architecture of the apical plasma membrane of epithelial cells and its dynamics. The latter is illustrated by the budding and fission of prominin-1-containing membrane vesicles from apical plasma membrane protrusions, which is controlled, at least in part, by the level of membrane cholesterol and the cholesterol-dependent organization of membrane microdomains.
极化上皮细胞的顶端质膜由不同的亚结构域组成，即平面区域和突起（微绒毛、初级纤毛），每个亚结构域都由特定的膜微结构域构建而成。包含五跨膜糖蛋白prominin-1和某些膜脂（特别是胆固醇）的组装体是顶端膜突起中这些微结构域的特征。在此，我们重点介绍了关于上皮细胞顶端质膜的分子结构及其动力学的最新研究发现。后者通过含prominin-1的膜囊泡从顶端质膜突起处出芽和裂变得到体现，这至少部分受膜胆固醇水平和膜微结构域的胆固醇依赖性组织的控制。
