Kasai K, Oda Y, Nishikata M, Ishii S
Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan.
J Chromatogr. 1986 Apr 11;376:33-47. doi: 10.1016/s0378-4347(00)80822-1.
Affinity chromatography is very useful in the investigation and characterization of specific interaction between biomolecules. Frontal analysis in affinity chromatography is advantageous from both theoretical and experimental viewpoints. The theory is very simple because we can describe this system by means of a simple equilibrium problem.Chromatographic data can be related easily to the amount of interacting molecules and the equilibrium constant. Useful equations analogous to those of enzyme kinetics can also be derived easily. Thus, frontal affinity chromatography provides information almost identical to that obtainable by enzyme kinetic studies. In addition, this method is more general because it does not depend on enzymatic activity. Experiment is very easy and does not require any special equipment. It is a powerful tool, especially for complicated systems where it has been difficult to find an appropriate method.
亲和色谱法在生物分子间特异性相互作用的研究与表征中非常有用。亲和色谱法中的前沿分析从理论和实验角度来看都具有优势。该理论非常简单，因为我们可以通过一个简单的平衡问题来描述这个系统。色谱数据能够轻松地与相互作用分子的量以及平衡常数相关联。类似于酶动力学的有用方程也能够很容易地推导出来。因此，前沿亲和色谱法提供的信息几乎与通过酶动力学研究所获得的信息相同。此外，这种方法更具通用性，因为它不依赖于酶活性。实验非常简单，不需要任何特殊设备。它是一种强大的工具，尤其适用于难以找到合适方法的复杂系统。
