猪流行性腹泻病毒胰酶诱导的血凝活性。
Trypsin-induced hemagglutination activity of porcine epidemic diarrhea virus.
机构信息
Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea.
出版信息
Arch Virol. 2010 Apr;155(4):595-9. doi: 10.1007/s00705-010-0620-5. Epub 2010 Mar 9.
Abstract
The hemagglutination (HA) activity of porcine epidemic diarrhea virus (PEDV) was investigated. Two cell-adapted strains of PEDV (KPEDV-9 and SM98LVec) were subjected to HA test against erythrocytes of various origin. Both strains showed HA activity with rabbit erythrocytes only after treatment with trypsin or neuraminidase. Optimal conditions for inducing HA activity of PEDV were 2 h incubation at 37 degrees C using phosphate-buffered saline containing 0.1% BSA. These results suggest that the HA activity of PEDV is most likely caused by proteolytic action on it, which could be developed as a new diagnostic method to rapidly detect and differentiate PEDV infections from other enteric diseases.
摘要
本研究调查了猪流行性腹泻病毒（PEDV）的血凝（HA）活性。对两种细胞适应株的PEDV（KPEDV-9 和 SM98LVec）进行了针对各种来源的红细胞的 HA 试验。只有在用胰蛋白酶或神经氨酸酶处理后，这两种株均显示出对兔红细胞的 HA 活性。诱导 PEDV 的 HA 活性的最佳条件是在含有 0.1％BSA 的磷酸盐缓冲盐水（PBS）中于 37°C 孵育 2 小时。这些结果表明，PEDV 的 HA 活性很可能是由其对蛋白酶的作用引起的，这可能被开发为一种新的诊断方法，用于快速检测和区分 PEDV 感染与其他肠道疾病。
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