Department of Anatomy a Morphological Neuroscience, Shimane University School of Medicine, Izumo, Japan.
Adv Exp Med Biol. 2010;669:189-92. doi: 10.1007/978-1-4419-5692-7_38.
The synaptic organization between vesicular glutamate transporter 2 (VGLUT2)-immunoreactive (ir) axon terminals and phrenic motoneurons in the neonatal rat was examined using a combined retrograde tracing and immunohistochemistry for VGLUT2. The phrenic nucleus (PhN) contained large numbers of VGLUT2-ir axon terminals, some of which made axosomatic and axodendritic synapses with PhN motoneurons. These terminals were of asymmetrical type and contained spherical clear synaptic vesicles. The results suggest that in the neonatal rat glutamatergic synapses onto PhN motoneurons exist and mediate excitatory transmission to drive PhN motoneurons.
使用逆行示踪和 VGLUT2 免疫组织化学方法研究了新生大鼠中囊泡谷氨酸转运体 2 (VGLUT2)-免疫反应性 (ir) 轴突末梢与膈神经运动神经元之间的突触组织。膈神经核 (PhN) 包含大量 VGLUT2-ir 轴突末梢，其中一些与 PhN 运动神经元形成轴体和轴突树突突触。这些末梢为不对称型，含有球形透明的突触小泡。结果表明，在新生大鼠中，谷氨酸能突触存在于 PhN 运动神经元上，并介导兴奋性传递以驱动 PhN 运动神经元。
