聚糖微阵列的生产与应用。

Production and application of glycan microarrays.

作者信息

Busch Julia, McBride Ryan, Head Steven R

机构信息

DNA Array Core Facility, The Scripps Research Institute, La Jolla, CA, USA.

出版信息

Methods Mol Biol. 2010;632:269-82. doi: 10.1007/978-1-60761-663-4_17.

DOI:10.1007/978-1-60761-663-4_17

PMID:20217584

Abstract

Glycans are vital elements of living organisms and are involved in recognition, communication, cell growth and development, motility, and other significant processes. The interactions of glycans with the proteins that bind them provide valuable information about protein interaction and specificity. By printing glycans on microarrays, investigators are able to effectively determine the binding specificity of certain proteins with an extremely efficient and precise result. Such chips are performed by standard robotic microarray printing. Incubating the slides with various GBP-containing substances not only reveals clear receptor preferences of the proteins, but also detects minute differences in structure specificity.

摘要

聚糖是生物体的重要组成部分，参与识别、通讯、细胞生长与发育、运动及其他重要过程。聚糖与结合它们的蛋白质之间的相互作用提供了有关蛋白质相互作用和特异性的有价值信息。通过在微阵列上打印聚糖，研究人员能够以极其高效和精确的结果有效确定某些蛋白质的结合特异性。此类芯片通过标准的机器人微阵列打印来制作。用各种含GBP的物质孵育载玻片，不仅能揭示蛋白质明确的受体偏好，还能检测结构特异性的细微差异。