表面等离子体共振生物传感器作为筛选朊病毒蛋白结合剂以寻找潜在抗朊病毒先导化合物的工具。

SPR biosensor as a tool for screening prion protein binders as potential antiprion leads.

作者信息

Chen Beining

机构信息

Department of Chemistry, University of Sheffield, Sheffield, UK.

出版信息

Methods Mol Biol. 2010;627:147-55. doi: 10.1007/978-1-60761-670-2_9.

DOI:10.1007/978-1-60761-670-2_9

PMID:20217619

Abstract

Prion diseases, also called transmissible spongiform encephalopathies (TSEs), are a group of neurodegenerative disorders affecting animals and humans. No effective treatments are currently available for the diseases, vCJD in particular. It is believed that the formation of protease-resistant insoluble prion protein (PrP(Sc)), which is the main component of amyloidal deposits, from the cellular prion protein (PrP(C)), is essential for the progression of the disease. Therefore, both PrP(Sc) and PrP(C) are currently being used as potential drug targets.This protocol details an optimised experimental protocol to conduct an affinity screening of compound libraries by the immobilisation of PrP(C) using an SPR-based instrument, Biacore 3000.

摘要

朊病毒疾病，也称为传染性海绵状脑病（TSEs），是一组影响动物和人类的神经退行性疾病。目前尚无针对这些疾病的有效治疗方法，尤其是变异型克雅氏病（vCJD）。据信，由细胞朊蛋白（PrP(C)）形成抗蛋白酶的不溶性朊病毒蛋白（PrP(Sc)）是淀粉样沉积物的主要成分，这对疾病的进展至关重要。因此，PrP(Sc)和PrP(C)目前都被用作潜在的药物靶点。本方案详细介绍了一种优化的实验方案，用于使用基于表面等离子体共振（SPR）的仪器Biacore 3000固定PrP(C)，对化合物库进行亲和筛选。