从普通海鸥（Larus canus）分离出的高致病性禽流感病毒H5N1亚型的特性

[Characteristic of high pathogenic avian influenza virus subtype H5N1 isolated from common gull (Larus canus)].

作者信息

Sharshov K A, Kurskaia O G, Zaĭkovskaia A V, Iurlov A K, Susloparov I M, Ternovoĭ V A, Durymanov A G, Zolotykh S I, Shestopalov A M, Drozdov I G

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2010 Jan-Feb(1):29-32.

PMID:20218341

Abstract

AIM

To study biological characteristics of H5N1 influenza virus isolated from common gull on south of West Siberia in 2006.

MATERIALS AND METHODS

Isolation and characterization of biological characteristics performed according to recommendations of World Health Organization.

RESULTS

Influenza virus A (H5N1) was first isolated from common gull (Larus canus) in Russia. Antigen of isolated virus had significant affinity to polyclonal sera obtained against high pathogenic avian influenza viruses H5N1 circulating in South-East Asia. Phylogenetic analysis of isolated strain revealed its belonging to group of Qinghai-related variants of H5N1 influenza virus. Aminoacid structure of hemagglutinin proteolytic cleavage site is characteristic for type A high pathogenicity avian influenza viruses. Experimental infection of chickens demonstrated high pathogenicity of the isolated virus.

CONCLUSION

Involvement of common gulls in circulation of subtype H5N1 influenza virus is demonstrated for the first time. Important role of species from Laridae family in unprecedented spreading of H5N1 influenza virus started in 2005 is discussed.

摘要

目的

研究2006年从西西伯利亚南部的普通鸥分离出的H5N1流感病毒的生物学特性。

材料与方法

按照世界卫生组织的建议进行生物学特性的分离和鉴定。

结果

在俄罗斯首次从普通鸥（黑头鸥）中分离出甲型流感病毒（H5N1）。分离出的病毒抗原与针对在东南亚传播的高致病性禽流感病毒H5N1获得的多克隆血清具有显著亲和力。对分离株的系统发育分析表明，它属于H5N1流感病毒青海相关变种组。血凝素蛋白水解裂解位点的氨基酸结构是甲型高致病性禽流感病毒的特征。鸡的实验性感染证明了分离出的病毒具有高致病性。