Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4236, USA.
Differentiation. 2010 Jul;80(1):1-8. doi: 10.1016/j.diff.2010.02.003. Epub 2010 Mar 10.
A phylogenetic analysis of the basic helix-loop-helix (bHLH) gene superfamily was performed using seven different species (human, mouse, rat, worm, fly, yeast, and plant Arabidopsis) and involving over 600 bHLH genes (Stevens et al., 2008). All bHLH genes were identified in the genomes of the various species, including expressed sequence tags, and the entire coding sequence was used in the analysis. Nearly 15% of the gene family has been updated or added since the original publication. A super-tree involving six clades and all structural relationships was established and is now presented for four of the species. The wealth of functional data available for members of the bHLH gene superfamily provides us with the opportunity to use this exhaustive phylogenetic tree to predict potential functions of uncharacterized members of the family. This phylogenetic and genomic analysis of the bHLH gene family has revealed unique elements of the evolution and functional relationships of the different genes in the bHLH gene family.
使用来自 7 个不同物种（人类、老鼠、大鼠、线虫、果蝇、酵母和植物拟南芥）的超过 600 个 bHLH 基因（Stevens 等人，2008 年）对基本螺旋-环-螺旋（bHLH）基因超家族进行了系统发育分析。在各种物种的基因组中都鉴定出了所有的 bHLH 基因，包括表达序列标签，并在分析中使用了整个编码序列。自最初发表以来，该基因家族的近 15%已经得到更新或添加。建立了一个涉及六个分支和所有结构关系的超级树，现在为其中的四个物种呈现。bHLH 基因超家族成员提供的丰富功能数据使我们有机会使用这个详尽的系统发育树来预测该家族未被表征成员的潜在功能。对 bHLH 基因家族的系统发育和基因组分析揭示了 bHLH 基因家族中不同基因的进化和功能关系的独特元素。
