Cao Xiaohong, Wang Aihua, Wang Chunling, Lu Meifang, Jiao Runzhi, Zhu Hui, Zhao Sisi
College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Nov;25(11):1705-10.
We studied the effect of surfactin on cell proliferation, apoptosis and the cytoskeleton in human breast cancer cell line MCF-7 in vitro. The result of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) showed that the surfactin inhibited proliferation of MCF-7 cells in a dose- and time-dependent manner, with IC50 at 48 h of 27.3 micromol/L. Surfactin-induced cell death was considered to be apoptotic by observing the typical apoptotic morphological changes by AO/EB staining. Flow cytometric analysis also demonstrated that surfactin caused time-dependent apoptosis of MCF-7 cells through cell arrest at G2/M phase. Immunofluorescence and Western blotting showed that surfactin significantly suppressed the expression of vimentin, induced the alpha-tubulin depolymerization and rearrangement and then the skeleton system of the cells changed dramatically. Based on our findings, surfactin can significantly inhibit the growth of MCF-7 cells and induce apoptosis.
我们在体外研究了表面活性素对人乳腺癌细胞系MCF-7细胞增殖、凋亡及细胞骨架的影响。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）实验结果表明，表面活性素以剂量和时间依赖性方式抑制MCF-7细胞的增殖，48小时的半数抑制浓度（IC50）为27.3微摩尔/升。通过吖啶橙/溴化乙锭（AO/EB）染色观察到典型的凋亡形态变化，表明表面活性素诱导的细胞死亡为凋亡。流式细胞术分析也表明，表面活性素通过使MCF-7细胞停滞于G2/M期而导致其时间依赖性凋亡。免疫荧光和蛋白质印迹显示，表面活性素显著抑制波形蛋白的表达，诱导α-微管蛋白解聚和重排，进而使细胞骨架系统发生显著变化。基于我们的研究结果，表面活性素可显著抑制MCF-7细胞的生长并诱导其凋亡。
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