Peighami-Ashnaei S, Sharifi-Tehrani A, Ahmadzadeh M, Behboudi K
Department of Plant Protection, Faculty of Horticultural Science & Plant Protection University of Tehran, Karaj, Iran.
Commun Agric Appl Biol Sci. 2009;74(3):745-8.
In this study fifteen isolates of identified Pseudomonas fluorescens and Bacillus subtilis were investigated for control of bean damping-off disease caused by Rhizoctonia solani. In vitro, P. fluorescens P-6 and B. subtilis B-3 showed the most inhibitory zone in dual culture assay against R. solani. The growth of P-6 (4.5 x 10(8) cfu/ml) was significantly higher than in the other treatments. In greenhouse condition, all of the isolates effectively controlled damping-off on bean. P. fluorescens P-5 and P-6 showed the considerable results against R. solani and could reduce the damping-off disease from 100% to less than 30%. P-5, P-6 (P. fluorescens) and 8-16 (8. subtilis) strains had the highest effect on fresh weight of bean.
在本研究中，对15株已鉴定的荧光假单胞菌和枯草芽孢杆菌菌株进行了研究，以控制由立枯丝核菌引起的菜豆猝倒病。在体外，荧光假单胞菌P-6和枯草芽孢杆菌B-3在与立枯丝核菌的双培养试验中显示出最大的抑菌圈。P-6（4.5×10⁸ cfu/ml）的生长显著高于其他处理。在温室条件下，所有分离株均能有效控制菜豆的猝倒病。荧光假单胞菌P-5和P-6对立枯丝核菌显示出显著效果，可将猝倒病发病率从100%降低至30%以下。P-5、P-6（荧光假单胞菌）和8-16（枯草芽孢杆菌）菌株对菜豆鲜重的影响最大。
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