核糖体DNA基因间隔区的亚重复序列在绿豆中作为显著的独立卫星DNA存在，但在赤小豆中则不存在。

Subrepeats of rDNA intergenic spacer present as prominent independent satellite DNA in Vigna radiata but not in Vigna angularis.

作者信息

Unfried K, Schiebel K, Hemleben V

机构信息

Lehrstuhl für Allgemeine Genetik, Biologisches Institut, Universität Tübingen, F.R.G.

出版信息

Gene. 1991 Mar 1;99(1):63-8. doi: 10.1016/0378-1119(91)90034-9.

DOI:10.1016/0378-1119(91)90034-9

PMID:2022324

Abstract

Subrepeats located in the rDNA intergenic spacer are also present as independently occurring, tandemly arranged satellite DNA clusters in the genome of Vigna radiata (mung bean). These 174-bp satellite repeats are identified as non-rDNA repeats by the presence of an AluI site. In the closely related Vigna angularis (adzuki bean), 174-bp repeats characterized by an AluI site occur in the rDNA with high sequence homology to the V. radiata rDNA subrepeats. A part of the 174-bp element that shows high similarity to a Xenopus terminator box (T2/T3) is slightly modified in V. angularis. However, a characteristic stem-loop structure can be formed, as in the case of V. radiata. Two highly conserved 12-bp regions occur within the 174-bp rDNA repeats of the two plants investigated. One of these 12-bp stretches exhibits some sequence identity to an element repeated twice in the 325-bp repeats in the intergenic spacer region of Vicia faba (broad bean).

摘要

位于核糖体DNA基因间隔区的亚重复序列，在绿豆（Vigna radiata）基因组中也以独立出现、串联排列的卫星DNA簇形式存在。这些174碱基对的卫星重复序列因存在一个AluI位点而被鉴定为非核糖体DNA重复序列。在近缘的赤豆（Vigna angularis）中，具有AluI位点特征的174碱基对重复序列出现在核糖体DNA中，与绿豆核糖体DNA亚重复序列具有高度序列同源性。174碱基对元件中与非洲爪蟾终止子框（T2/T3）高度相似的部分，在赤豆中略有修饰。然而，与绿豆情况一样，可以形成一个特征性的茎环结构。在所研究的两种植物的174碱基对核糖体DNA重复序列中存在两个高度保守的12碱基对区域。其中一个12碱基对片段与蚕豆（Vicia faba）基因间隔区325碱基对重复序列中重复两次的一个元件具有一些序列一致性。