Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, Changsha, Hunan 410078, China.
In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):192-9. doi: 10.1007/s11626-010-9307-1. Epub 2010 Mar 12.
To establish a potential resource for cell therapy and a developmental model for human diseases, we had isolated three Chinese human embryonic stem cell lines from the inner cell mass of human blastocysts in 2002. All the three cell lines were grown on mouse embryonic fibroblasts as feeder cells; one of these cell lines, chHES-3, has maintained its normal karyotype even after being cultured in vitro for more than 100 passages, after the standardization of mouse feeder preparation. Each hES cell line has been completely characterized. All the three cell lines expressed hES-specific markers and pluripotency-related genes. These cells maintained their normal karyotype during long-term culture and displayed a high telomerase activity. When differentiated in vivo and in vitro, the derivatives representing the three germ layers could be observed. Human leukocyte antigen, ABO blood type, and DNA fingerprinting were also performed to provide a unique identity to each cell line. By establishing these hES cell lines, we provide an appropriate in vitro model to study human development and regeneration. All the three cell lines can be obtained for research purposes by placing a request at our website at www.hescbank.cn.
为建立细胞治疗的潜在资源和人类疾病的发育模型，我们于 2002 年从人类囊胚的内细胞团中分离出三株中国来源的人胚胎干细胞系。所有这三种细胞系均在饲养层细胞（小鼠胚胎成纤维细胞）上生长；其中一个细胞系 chHES-3，在经过标准化的饲养层制备后，即使在体外培养超过 100 代后，仍保持正常核型。每株 hES 细胞系都经过了全面的特征鉴定。所有三种细胞系均表达 hES 特异性标志物和多能性相关基因。这些细胞在长期培养过程中保持正常核型，并显示出高端粒酶活性。在体内和体外分化时，可以观察到代表三个胚层的衍生物。还进行了人类白细胞抗原、ABO 血型和 DNA 指纹分析，为每个细胞系提供了独特的身份。通过建立这些 hES 细胞系，我们提供了一个合适的体外模型来研究人类的发育和再生。通过访问我们的网站 www.hescbank.cn 提出申请，即可获得这三种细胞系用于研究目的。
