强力霉素调控的慢病毒载体。

Doxycycline regulated lentiviral vectors.

作者信息

Markusic David, Seppen Jurgen

机构信息

AMC Liver Center, Amsterdam, The Netherlands.

出版信息

Methods Mol Biol. 2010;614:69-76. doi: 10.1007/978-1-60761-533-0_4.

DOI:10.1007/978-1-60761-533-0_4

PMID:20225036

Abstract

Lentiviral vectors are a powerful tool to achieve regulated expression of transgenes in vivo and in vitro. The doxycycline-inducible system is well characterized and can be used to regulate expression mediated by lentiviral vectors. Because many different doxycycline-inducible lentiviral vectors have been described, choosing the best vector system can be difficult. This chapter can be used as a guide to select the optimal system for a particular application.

摘要

慢病毒载体是在体内和体外实现转基因调控表达的有力工具。强力霉素诱导系统已得到充分表征，可用于调控慢病毒载体介导的表达。由于已经描述了许多不同的强力霉素诱导型慢病毒载体，因此选择最佳载体系统可能会很困难。本章可作为为特定应用选择最佳系统的指南。