Cammack R, Rao K K, Bargeron C P, Hutson K G, Andrew P W, Rogers L J
Biochem J. 1977 Nov 15;168(2):205-9. doi: 10.1042/bj1680205.
Midpoint potentials of plant-type ferredoxins from a range of sources were measured by redox titrations combined with electron-paramagnetic-resonance spectroscopy. For ferredoxins from higher plants, green algae and most red algae, the midpoint potentials (at pH 8.0) were between --390 and --425 mV. Values for the major ferredoxin fractions from blue-green algae were less negative (between --325 and --390 mV). In addition, Spirulina maxima and Nostoc strain MAC contain second minor ferredoxin components with a different potential, --305 mV (the highest so far measured for a plant-algal ferrodoxin) for Spirulina ferrodoxin II, and --455 mV (the lowest so far measured for a plant-algal ferredoxin) for Nostoc strain MAC ferredoxin II. However, two ferredoxins extracted from a variety of the higher plant Pisum sativum (pea) had midpoint potentials that were only slightly different from each other. These values are discussed in terms of possible roles for the ferredoxins in addition to their involvement in photosynthetic electron transport.
通过氧化还原滴定结合电子顺磁共振光谱法，测量了来自一系列来源的植物型铁氧化还原蛋白的中点电位。对于高等植物、绿藻和大多数红藻中的铁氧化还原蛋白，中点电位（在pH 8.0时）在-390至-425毫伏之间。蓝藻主要铁氧化还原蛋白组分的值较正（在-325至-390毫伏之间）。此外，极大螺旋藻和念珠藻MAC菌株含有第二种次要的铁氧化还原蛋白组分，其电位不同，极大螺旋藻铁氧化还原蛋白II为-305毫伏（这是迄今为止测量的植物-藻类铁氧化还原蛋白的最高值），念珠藻MAC菌株铁氧化还原蛋白II为-455毫伏（这是迄今为止测量的植物-藻类铁氧化还原蛋白的最低值）。然而，从多种高等植物豌豆中提取的两种铁氧化还原蛋白的中点电位彼此仅略有不同。除了参与光合电子传递外，还根据铁氧化还原蛋白的可能作用对这些值进行了讨论。
