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采用新型表面印迹技术制备尿酸印迹材料及其识别性能研究。

Preparation and recognition performance of uric acid-imprinted material prepared with novel surface imprinting technique.

机构信息

Department of Chemical Engineering, North University of China, Taiyuan 030051, China.

出版信息

J Chromatogr A. 2010 Apr 9;1217(15):2226-36. doi: 10.1016/j.chroma.2010.02.027. Epub 2010 Feb 20.

Abstract

Acrylonitrile (AN) was first graft-polymerized on the surfaces of crosslinked polyvinyl alcohol (CPVA) microspheres by initiating of cerium salt, and then the grafted polyacrylonitrile (PAN) was transformed to polyamidoxime (PAO) via amidoximation transform reaction, resulting in the functional microspheres PAO/CPVA. By adopting the novel surface-molecular imprinting technique put forward by us, uric acid molecule-imprinted material MIP-PAO/CPVA was prepared with glutaraldehyde as crosslinking agent The binding character of MIP-PAO/CPVA towards uric acid was investigated in depth with both batch and column methods and using guanine as a contrast substance whose chemical structure is similar to uric acid to a certain extent. The experimental results show that the surface imprinted material MIP-PAO/CPVA has excellent binding affinity (a great binding capacity of 104 mg/g) and high recognition selectivity for the template molecule, uric acid. The selectivity coefficient of PAO/CPVA microspheres (non-imprinted material) for uric acid relative to guanine is only 1.273, displaying no recognition selectivity for uric acid. However, after imprinting, the selectivity coefficient of MIP-PAO/CPVA for uric acid in respect to guanine is remarkably enhanced to 14.00, displaying the excellent recognition selectivity and binding affinity towards uric acid molecules.

摘要

丙烯腈 (AN) 首先通过铈盐引发在交联聚乙烯醇 (CPVA) 微球表面接枝聚合,然后将接枝的聚丙烯腈 (PAN) 通过羟胺化转化反应转化为聚酰胺氧 (PAO),得到功能化微球 PAO/CPVA。采用我们提出的新型表面分子印迹技术,以戊二醛为交联剂,制备了尿酸分子印迹材料 MIP-PAO/CPVA。采用间歇法和柱法,以鸟嘌呤为对比物质(其化学结构在一定程度上与尿酸相似),深入研究了 MIP-PAO/CPVA 对尿酸的结合特性。实验结果表明,表面印迹材料 MIP-PAO/CPVA 对模板分子尿酸具有优异的结合亲和力(结合容量高达 104mg/g)和高识别选择性。PAO/CPVA 微球(非印迹材料)对尿酸相对于鸟嘌呤的选择性系数仅为 1.273,对尿酸没有识别选择性。然而,印迹后,MIP-PAO/CPVA 对尿酸相对于鸟嘌呤的选择性系数显著提高到 14.00,显示出对尿酸分子的优异识别选择性和结合亲和力。

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