水合哈尔明对猴小肠氨基酸和二肽转运的钠依赖性抑制作用。

Sodium-dependent inhibition of amino acid and dipeptide transport by harmaline in monkey small intestine.

机构信息

School of Life Sciences, University of Hyderabad, Hyderabad 500 001, India.

出版信息

Biochem Pharmacol. 1980 Mar 1;29(5):713-6. doi: 10.1016/0006-2952(80)90545-6.

PMID:20227945

Abstract

The effect of harmaline on amino acid and dipeptide uptake was studied in the monkey. Harmaline inhibited both the systems. Preincubation of the tissue with 4 mmole/1 harmaline for 10 min was necessary for maximal inhibition. The uptake of glycyl-l-leucine- ((14)C) and ((14)C)-glycyl-l-leucine was inhibited to the same extent. The uptake of glycyl-l-proline, a dipeptide which was presumably taken up intact by the mucosal cells, was also inhibited. Total replacement of sodium in the medium reduced the uptake of both glycyl-l-leucine and glycyl-l-proline. But the uptake of these dipeptides was not inhibited by harmaline in the absence of sodium. However, the sodium-independent uptake of glycyl-l-leucine and glycyl-l-proline was also inhibited by other dipeptides.

摘要

研究了哈马林对猴子中氨基酸和二肽摄取的影响。哈马林抑制了这两个系统。组织在 4mmole/1 哈马林中预孵育 10 分钟是最大抑制所必需的。甘氨酰-l-亮氨酸-((14)C)和((14)C)-甘氨酰-l-亮氨酸的摄取受到相同程度的抑制。甘氨酰-l-脯氨酸，一种假定被粘膜细胞完整摄取的二肽，其摄取也被抑制。介质中钠离子的完全替代减少了甘氨酰-l-亮氨酸和甘氨酰-l-脯氨酸的摄取。但是，在没有钠离子的情况下，哈马林并没有抑制这些二肽的摄取。然而，甘氨酰-l-亮氨酸和甘氨酰-l-脯氨酸的钠离子非依赖性摄取也被其他二肽抑制。