School of Life Sciences, University of Hyderabad, Hyderabad 500 001, India.
Biochem Pharmacol. 1980 Mar 1;29(5):713-6. doi: 10.1016/0006-2952(80)90545-6.
The effect of harmaline on amino acid and dipeptide uptake was studied in the monkey. Harmaline inhibited both the systems. Preincubation of the tissue with 4 mmole/1 harmaline for 10 min was necessary for maximal inhibition. The uptake of glycyl-l-leucine- ((14)C) and ((14)C)-glycyl-l-leucine was inhibited to the same extent. The uptake of glycyl-l-proline, a dipeptide which was presumably taken up intact by the mucosal cells, was also inhibited. Total replacement of sodium in the medium reduced the uptake of both glycyl-l-leucine and glycyl-l-proline. But the uptake of these dipeptides was not inhibited by harmaline in the absence of sodium. However, the sodium-independent uptake of glycyl-l-leucine and glycyl-l-proline was also inhibited by other dipeptides.
研究了哈马林对猴子中氨基酸和二肽摄取的影响。哈马林抑制了这两个系统。组织在 4mmole/1 哈马林中预孵育 10 分钟是最大抑制所必需的。甘氨酰-l-亮氨酸-((14)C)和((14)C)-甘氨酰-l-亮氨酸的摄取受到相同程度的抑制。甘氨酰-l-脯氨酸，一种假定被粘膜细胞完整摄取的二肽，其摄取也被抑制。介质中钠离子的完全替代减少了甘氨酰-l-亮氨酸和甘氨酰-l-脯氨酸的摄取。但是，在没有钠离子的情况下，哈马林并没有抑制这些二肽的摄取。然而，甘氨酰-l-亮氨酸和甘氨酰-l-脯氨酸的钠离子非依赖性摄取也被其他二肽抑制。
