类风湿关节炎患者外周血Th17/Treg细胞比例失衡的研究

[Study on ratio imbalance of peripheral blood Th17/Treg cells in patients with rheumatoid arthritis].

作者信息

Niu Qian, Huang Zhuo-chun, Cai Bei, Wang Lan-lan, Feng Wei-hua

机构信息

Clinical Immunological Laboratory, West China Hospital of Sichuan University, Chengdu, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Mar;26(3):267-9, 272.

PMID:20230694

Abstract

AIM

To observe the relationship between balance of peripheral blood Th17 cells and Foxp3(+) CD4(+) CD25(+) regulatory T (Treg) cells in patients with rheumatoid arthritis (RA), and to clarify the role the ratio imbalance of peripheral blood Th17/Treg cells playing in pathogenesis of RA.

METHODS

The ratio of peripheral blood Th17 cells and Foxp3(+) CD4(+) CD25(+) Treg cells in RA patients and healthy subjects were determined by flow cytometry (FCM).

RESULTS

Compared with healthy controls, the ratio of both CD3(+) CD4(+) T cells and Th17 cells in RA patients increased significantly (P<0.05), while the percentage of Foxp3(+) CD4(+) CD25(+) Treg cells was markedly lower (P<0.05). With the development of RA activity, the ratio of Th17 cells increased (P<0.05), and the ratio of Foxp3(+) CD4(+) CD25(+) Treg cells decreased (P>0.05).

CONCLUSION

The disorder of peripheral blood T lymphocyte subsets in RA patients characterized by increased CD4(+) T cells. The imbalance between Th17 cells and Foxp3(+) CD4(+) CD25(+) Treg cells resulted from increased ratio of Th17 cells and decreased ratio of Foxp3(+) CD4(+) CD25(+) Treg cells may play a critical role in RA progression.

摘要

目的

观察类风湿关节炎（RA）患者外周血Th17细胞与Foxp3(+)CD4(+)CD25(+)调节性T细胞（Treg细胞）平衡之间的关系，阐明外周血Th17/Treg细胞比例失衡在RA发病机制中的作用。

方法

采用流式细胞术（FCM）检测RA患者和健康对照者外周血Th17细胞与Foxp3(+)CD4(+)CD25(+)Treg细胞的比例。

结果

与健康对照相比，RA患者CD3(+)CD4(+)T细胞和Th17细胞比例均显著升高（P<0.05），而Foxp3(+)CD4(+)CD25(+)Treg细胞百分比明显降低（P<0.05）。随着RA病情活动度的增加，Th17细胞比例升高（P<0.05），Foxp3(+)CD4(+)CD25(+)Treg细胞比例降低（P>0.05）。