中国慢性牙周炎患者 Toll 样受体 2、Toll 样受体 4、CSF2 和 LY64 的体内表达。
In vivo expression of Toll-like receptor 2, Toll-like receptor 4, CSF2 and LY64 in Chinese chronic periodontitis patients.
机构信息
Department of Periodontology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Oral Dis. 2010 May;16(4):343-50. doi: 10.1111/j.1601-0825.2009.01630.x. Epub 2010 Mar 9.
OBJECTIVE
Toll-like receptors (TLRs) are the essential components in the innate and adaptive immune systems. Colony stimulating factor 2 (CSF2) is a cytokine that may prevent endotoxin tolerance, and LY64 has the ability to interfere with the recognition of bacteria via TLR4. The aim of this study was to explore the in vivo expressions of TLR2, TLR4, CSF2 and LY64 in Chinese chronic periodontitis patients.
METHODS
Gingival biopsies were collected from 24 chronic periodontitis patients and 19 healthy controls. The gene expression profiles of TLR2, TLR4, CSF2 and LY64 were investigated by real-time polymerase chain reaction, and the protein expressions of TLR2 and TLR4 were detected by immunohistochemistry. In addition, the levels of CSF2 in gingival crevicular fluid (GCF) were determined by ELISA.
RESULTS
The higher mRNA expressions of TLR2, TLR4 and CSF2, and the lower mRNA expression of LY64 were detected in chronic periodontitis patients. And the increased protein expressions of TLR2 and TLR4 were confirmed by immunohistochemistry. In addition, the increase of total amount of CSF2 in GCF was observed in chronic periodontitis patients.
CONCLUSIONS
Our results suggest that TLR2 and TLR4 may play a role in periodontal pathogenesis. In addition, CSF2 and LY64 may contribute to the regulation of inflammatory response and maintaining periodontal homeostasis.
目的
Toll 样受体（TLRs）是先天和适应性免疫系统的重要组成部分。集落刺激因子 2（CSF2）是一种细胞因子，可能防止内毒素耐受，而 LY64 具有通过 TLR4 干扰细菌识别的能力。本研究旨在探讨中国慢性牙周炎患者体内 TLR2、TLR4、CSF2 和 LY64 的表达情况。
方法
从 24 例慢性牙周炎患者和 19 例健康对照者中采集牙龈活检组织。通过实时聚合酶链反应检测 TLR2、TLR4、CSF2 和 LY64 的基因表达谱，并通过免疫组织化学检测 TLR2 和 TLR4 的蛋白表达。此外，通过 ELISA 测定龈沟液（GCF）中 CSF2 的水平。
结果
慢性牙周炎患者 TLR2、TLR4 和 CSF2 的 mRNA 表达水平升高，LY64 的 mRNA 表达水平降低。免疫组织化学证实 TLR2 和 TLR4 的蛋白表达增加。此外，慢性牙周炎患者 GCF 中 CSF2 的总量增加。
结论
我们的结果表明 TLR2 和 TLR4 可能在牙周病发病机制中发挥作用。此外，CSF2 和 LY64 可能有助于调节炎症反应和维持牙周稳态。
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