Institute of Cell Biology and Centre for Science at Extreme Conditions, University of Edinburgh, UK.
Ann N Y Acad Sci. 2010 Feb;1189:143-8. doi: 10.1111/j.1749-6632.2009.05178.x.
The deep-sea bacterium, Photobacterium profundum SS9, has been adopted as a model organism to understand the molecular basis of cold-adapted high-pressure-loving (piezophilic) growth. Despite growing optimally at 28 MPa (15 degrees C), P. profundum SS9 can grow over a wide range of pressures and temperatures. The ability to grow at atmospheric pressure has enabled a limited set of genetic tools to be developed, which has provided genetic insights into the mechanism of piezophilic growth in P. profundum SS9. This review focuses on how genetic studies have uncovered the importance of processes affecting the DNA and the bacterial cell envelope in the piezophilic growth of P. profundum SS9. In addition, a method was developed to assess quantitative piezophilic colony growth of P. profundum SS9 on solid agar. Future studies, using this methodology, could provide novel insights into the molecular basis of piezophilic, surface-attached growth.
深海细菌 Photobacterium profundum SS9 被选为模式生物，以了解适应低温高压的（嗜压）生长的分子基础。尽管 P. profundum SS9 在 28 MPa（15 摄氏度）下生长最佳，但它可以在很宽的压力和温度范围内生长。能够在大气压下生长使开发出了一套有限的遗传工具，这为深入了解 P. profundum SS9 的嗜压生长机制提供了遗传上的认识。这篇综述重点介绍了遗传研究如何揭示影响 DNA 和细菌细胞包膜的过程在 P. profundum SS9 的嗜压生长中的重要性。此外，还开发了一种方法来评估 P. profundum SS9 在固体琼脂上定量嗜压菌落生长的情况。使用这种方法的未来研究可以为嗜压、表面附着生长的分子基础提供新的见解。
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