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[油菜籽(Brasscia napus L.)产量构成因素的数量性状基因座的分子定位与鉴定]

[Molecular mapping and identification of quantitative trait loci for yield components in rapeseed (Brasscia napus L.)].

作者信息

Wang Feng, Guan Chun-Yun

机构信息

Oilseed Crops Institute/National Oilseed Crops Improvement Center in Hunan, Hunan Agricultural University, Changsha 410128, China.

出版信息

Yi Chuan. 2010 Mar;32(3):271-7. doi: 10.3724/sp.j.1005.2010.00271.

Abstract

A F2 segregating population for genetic map construction and identification of QTL for seed yield in rapeseed (Brassica napus L.), was developed via crossing a conventional rapeseed line 04-1139 with a high yielding multiple silique rapeseed line 05-1054. A genetic map including 19 linkage groups was constructed with 200 SSR (Simple sequence repeat) and SRAP (Sequence-related amplified polymorphism) markers. This map covers a total length of 1 700.23 cM with an average distance between two adjacent makers of 8.50 cM. Using this map, QTL for the components of yield per plant, such as number of silique per plant (SNP), number of seeds per silique (SS) were analyzed. A total of 12 putative QTL for the traits were detected. Four of them were associated with SNP, which explained 35.64%, 12.96%, 28.71%, and 34.02% of the variation, respectively. Five QTL, which explained 8.41%, 7.87%, 24.37%, 8.57%, and 14.31% of the variation, were responsible for SS. Three QTL for 1 000-seed weight explained 1.81%-2.33% of the variation. The additional effects of the alleles for a trait may originate from both parents. The markers associated with the main QTL can be a good tool for marker associated selection and pyramiding breeding.

摘要

通过将常规油菜品系04 - 1139与高产多角果油菜品系05 - 1054杂交,构建了一个用于油菜(甘蓝型油菜L.)遗传图谱构建和种子产量QTL鉴定的F2分离群体。利用200个SSR(简单序列重复)和SRAP(序列相关扩增多态性)标记构建了包含19个连锁群的遗传图谱。该图谱全长1700.23 cM,相邻标记间平均距离为8.50 cM。利用此图谱,分析了单株产量构成因素的QTL,如单株角果数(SNP)、每角粒数(SS)。共检测到12个与这些性状相关的假定QTL。其中4个与SNP相关,分别解释了35.64%、12.96%、28.71%和34.02%的变异。5个与SS相关的QTL解释了8.41%、7.87%、24.37%、8.57%和14.31%的变异。3个与千粒重相关的QTL解释了1.81% - 2.33%的变异。一个性状等位基因的加性效应可能来自双亲。与主要QTL相关的标记可成为标记辅助选择和聚合育种的良好工具。

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