Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma, OK 73104, USA.
Adv Exp Med Biol. 2010;664:63-70. doi: 10.1007/978-1-4419-1399-9_8.
Retinal degeneration slow (RDS) is a photoreceptor specific tetraspanin membrane protein. It is expressed in the rim region of rod outer segment (OS) discs and cone OS lamellae. Mutations in RDS cause both rod and cone-dominant retinal degenerations. We have recently shown that RDS functions differently in rods vs. cones, and have used the cone-dominant nrl ( -/- ) and rod-dominant wild-type (WT) murine retinas to study these differences and help understand the mechanism of rod and cone OS biogenesis. We hypothesize that the differential role of RDS in rods vs. cones is in part related to differences in RDS binding partners. RDS has been shown to bind to the GARP portion of the beta subunit of the rod-cyclic nucleotide gated (CNG) channel. This interaction has been hypothesized to play a role in anchoring the disc rim to the rod plasma membrane. In this study we show that RDS does not interact with the cone CNG. Given that cone lamellae are not entirely encased in plasma membrane and therefore may have different anchoring requirements compared with rods, this observation may help explain some of the differential behavior of RDS in rods vs. cones.
视网膜退行性缓慢（RDS）是一种感光细胞特异性四跨膜蛋白。它在视杆外节（OS）盘的边缘区域和视锥 OS 薄片中表达。RDS 突变会导致视杆和视锥主导的视网膜变性。我们最近表明，RDS 在视杆细胞和视锥细胞中的功能不同，并使用视锥细胞主导的 nrl（-/-）和视杆细胞主导的野生型（WT）鼠视网膜来研究这些差异，以帮助理解视杆和视锥 OS 发生的机制。我们假设 RDS 在视杆细胞和视锥细胞中的差异作用部分与 RDS 结合伴侣的差异有关。已经表明 RDS 与视杆细胞环核苷酸门控（CNG）通道β亚基的 GARP 部分结合。这种相互作用被假设在将盘边缘锚定到视杆细胞膜上发挥作用。在这项研究中，我们表明 RDS 不会与视锥 CNG 相互作用。鉴于视锥薄片不完全被质膜包裹，因此与视杆细胞相比，可能具有不同的锚定要求，这一观察结果可能有助于解释 RDS 在视杆细胞和视锥细胞中的差异行为。
