一种新的低分子量类视黄醇结合蛋白。

A new retinoid-binding protein with a low molecular weight.

作者信息

Sani B P, Reddy L G, Singh R K, Arello E A

机构信息

Kettering-Meyer Laboratory, Southern Research Institute, Birmingham, Alabama 35255-5305.

出版信息

Biochem Biophys Res Commun. 1991 Mar 29;175(3):1064-9. doi: 10.1016/0006-291x(91)91673-z.

DOI:10.1016/0006-291x(91)91673-z

PMID:2025238

Abstract

Cellular retinoid-binding proteins and nuclear receptors may mediate the intracellular transport and the action of retinoids in the control of differentiation and tumorigenesis. We report a new retinoid-binding protein (Ret BP) with a molecular size of 4,000 that binds retinol, retinoic acid, and some of their derivatives. Purification of Ret BP from chick skin cytosol involved DEAE-Sephadex, Sephadex G-100, and Mono Q column chromatography. The Ret BP-retinoid complex eluted at 195 mM NaCl during Mono Q column chromatography using a 0-300 nM NaCl gradient. Superose-12 column chromatography indicated a molecular size of 4,000 for Ret BP. The binding protein showed a pI of 6.8 on electrofocusing in ampholines of pH 3-10. Ret BP may act as an affinant for retinoids in the cell, and may serve to dispense the ligands to their respective functionally active sites.

摘要

细胞视黄醇结合蛋白和核受体可能介导视黄醇在细胞内的运输以及视黄醇在分化和肿瘤发生控制中的作用。我们报道了一种新的视黄醇结合蛋白（Ret BP），其分子量为4000，能结合视黄醇、视黄酸及其一些衍生物。从鸡皮肤胞质溶胶中纯化Ret BP涉及DEAE-葡聚糖凝胶、葡聚糖凝胶G-100和Mono Q柱色谱法。在使用0-300 nM NaCl梯度的Mono Q柱色谱过程中，Ret BP-视黄醇复合物在195 mM NaCl处洗脱。Superose-12柱色谱表明Ret BP的分子量为4000。在pH 3-10的两性电解质中进行等电聚焦时，该结合蛋白的pI为6.8。Ret BP可能作为细胞内视黄醇的亲和剂，并可能用于将配体分配到其各自的功能活性位点。