对顺铂、阿霉素和左旋苯丙氨酸氮芥耐药的人卵巢癌细胞系对Δ7-前列腺素A1和Δ12-前列腺素J2敏感。

Human ovarian cancer cell lines resistant to cisplatin, doxorubicin, and L-phenylalanine mustard are sensitive to delta 7-prostaglandin A1 and delta 12-prostaglandin J2.

作者信息

Sasaki H, Takada K, Terashima Y, Ekimoto H, Takahashi K, Tsuruo T, Fukushima M

机构信息

Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.

出版信息

Gynecol Oncol. 1991 Apr;41(1):36-40. doi: 10.1016/0090-8258(91)90251-y.

DOI:10.1016/0090-8258(91)90251-y

PMID:2026357

Abstract

The antitumor activity of delta 7-prostaglandin A1 (delta 7-PGA1) or delta 12-prostaglandin J2 (delta 12-PGJ2) on human ovarian cancer cell lines resistant to cisplatin (CDDP), doxorubicin (ADR), and L-phenylalanine mustard (l-PAM) was studied in vitro. A2780AD, A2780 (parent cells of A2780AD), 2008DDP, and 2008 cells (parent cells of 2008DDP) were used. The antitumor activities of the drugs were defined with 50% inhibitory concentration (IC50) estimated from growth inhibition curves, which were obtained by an indirect colorimetric method. Drug-resistance ratios obtained from IC50 values, by comparing A2780AD and A2780 cells, were 62.5 for ADR, 4.6 for CDDP, 4.9 for l-PAM, 1.5 for delta 7-PGA1, and 1.8 for delta 12-PGJ2. Those obtained by comparing 2008DDP and 2008 cells were 1.1 for ADR, 16.0 for CDDP, 2.9 for l-PAM, 2.3 for delta 7-PGA1, and 3.2 for delta 12-PGJ2. Thus some human ovarian cancer cells resistant to ADR, CDDP, and l-PAM remain sensitive to antitumor PGs.

摘要

在体外研究了δ7-前列腺素A1（δ7-PGA1）或δ12-前列腺素J2（δ12-PGJ2）对顺铂（CDDP）、阿霉素（ADR）和L-苯丙氨酸氮芥（l-PAM）耐药的人卵巢癌细胞系的抗肿瘤活性。使用了A2780AD、A2780（A2780AD的亲本细胞）、2008DDP和2008细胞（2008DDP的亲本细胞）。通过间接比色法获得生长抑制曲线，根据该曲线估算50%抑制浓度（IC50）来确定药物的抗肿瘤活性。通过比较A2780AD和A2780细胞的IC50值得到的耐药比，ADR为62.5，CDDP为4.6，l-PAM为4.9，δ7-PGA1为1.5，δ12-PGJ2为1.8。通过比较2008DDP和2008细胞得到的耐药比，ADR为1.1，CDDP为16.0，l-PAM为2.9，δ7-PGA1为2.3，δ12-PGJ2为3.2。因此，一些对ADR、CDDP和l-PAM耐药的人卵巢癌细胞对抗肿瘤前列腺素仍敏感。