牛巴贝斯虫表达一种对中和敏感的抗原，该抗原含有微线体黏附重复（MAR）结构域。
Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain.
作者信息
Silva Marta G, Ueti Massaro W, Norimine Junzo, Florin-Christensen Monica, Bastos Reginaldo G, Goff Will L, Brown Wendy C, Oliva Abel, Suarez Carlos E
机构信息
Biomolecular Diagnostic Laboratory, ITQB/IBET, Universidade Nova de Lisboa, Av. da Republica, Apt 12, Oeiras, Portugal.
出版信息
Parasitol Int. 2010 Jun;59(2):294-7. doi: 10.1016/j.parint.2010.03.004. Epub 2010 Mar 19.
A gene coding for a protein with sequence similarity to the Toxoplasma gondii micronemal 1 (MIC1) protein that contains a copy of a domain described as a sialic acid-binding micronemal adhesive repeat (MAR) was identified in the Babesia bovis genome. The single copy gene, located in chromosome 3, contains an open reading frame encoding a putative 181 amino acid protein, which is highly conserved among distinct B. bovis strains. Antibodies against both recombinant protein and synthetic peptides mimicking putative antigenic regions in the B. bovis-MIC1 (Bbo-MIC1) protein bind to the parasite in immunofluorescence assays and significantly inhibit erythrocyte invasion in in vitro B. bovis cultures. Bbo-MIC1 is recognized by antibodies in serum from B. bovis infected cattle, demonstrating expression and immunogenicity during infection. Overall, the results suggest that Bbo-MIC1 protein is a viable candidate for development of subunit vaccines.
在牛巴贝斯虫基因组中鉴定出一个编码与刚地弓形虫微小膜体蛋白1（MIC1）具有序列相似性的蛋白质的基因，该蛋白质含有一个被描述为唾液酸结合微小膜体黏附重复序列（MAR）的结构域拷贝。这个位于3号染色体上的单拷贝基因包含一个开放阅读框，编码一个推定的181个氨基酸的蛋白质，该蛋白质在不同的牛巴贝斯虫菌株中高度保守。在免疫荧光试验中，针对重组蛋白和模拟牛巴贝斯虫-MIC1（Bbo-MIC1）蛋白推定抗原区域的合成肽的抗体与寄生虫结合，并在体外牛巴贝斯虫培养物中显著抑制红细胞入侵。Bbo-MIC1被牛巴贝斯虫感染牛血清中的抗体识别，表明其在感染期间表达且具有免疫原性。总体而言，结果表明Bbo-MIC1蛋白是亚单位疫苗开发的一个可行候选物。
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