Ascencio F, Ljungh A, Wadström T
Department of Medical Microbiology, University of Lund, Sweden.
Microbios. 1991;65(264-265):135-46.
Binding of 125I-labelled types I and IV collagen, fibronectin and laminin to Aeromonas cells is a common characteristic among Aeromonas strains isolated from diseased fish and human infections. The proportion of Aeromonas strains which bind to the various proteins were significantly greater for A. hydrophila than for A. sobria or A. caviae. The binding property was specific since it was inhibited by unlabelled homologous proteins. Bacterial cells incubated with proteolytic enzymes lose the ability of binding to 125I-labelled collagen type I and IV, fibronectin, laminin and vitronectin. A bacterial cell surface protein extract, containing active receptors competes with intact cells for 125I-extracellular matrix protein binding. Culture conditions greatly influence the expression of A. hydrophila cell surface binding structures for extracellular matrix proteins. The presence of calcium ions in the growth medium seems to be an important enhancer of the expression of extracellular matrix protein cell surface receptors.
125I标记的I型和IV型胶原蛋白、纤连蛋白和层粘连蛋白与气单胞菌细胞的结合是从患病鱼类和人类感染中分离出的气单胞菌菌株的共同特征。嗜水气单胞菌结合各种蛋白质的菌株比例显著高于温和气单胞菌或豚鼠气单胞菌。这种结合特性具有特异性，因为它会被未标记的同源蛋白质抑制。用蛋白水解酶孵育的细菌细胞失去了与125I标记的I型和IV型胶原蛋白、纤连蛋白、层粘连蛋白和玻连蛋白结合的能力。含有活性受体的细菌细胞表面蛋白提取物与完整细胞竞争125I-细胞外基质蛋白的结合。培养条件极大地影响嗜水气单胞菌细胞表面与细胞外基质蛋白结合结构的表达。生长培养基中钙离子的存在似乎是细胞外基质蛋白细胞表面受体表达的重要增强剂。
