Tonew E M
Virologie. 1977 Oct-Dec;28(4):295-8.
Virus-specific RNA polymerase activity of mengovirus-infected Ehrlich ascites carcinoma (EAC) cells was maximal 7 hours after infection in a one-step growth cycle. In a cell-free system with the mitochondrial-microsomal fraction (MMF) derived from mengovirus-infected EAC cells in a nucleoside-triphosphate medium, polymerase activity increased up to 60 min (when MMF was obtained by a homogenizer) or 120 min (when MMF was prepared by sodium deoxycholate disruption). Subsequently RNA polymerase activity decreased between 150-180 min of incubation at 37 degrees C and then formed a plateau up to 300 min. The cell-free system used is able to give valuable information as regards testing of RNA polymerase inhibitors in an antiviral screening programme.
在一步生长周期中，感染脑心肌炎病毒的艾氏腹水癌（EAC）细胞的病毒特异性RNA聚合酶活性在感染后7小时达到最大值。在核苷三磷酸培养基中，使用源自感染脑心肌炎病毒的EAC细胞的线粒体-微粒体组分（MMF）的无细胞系统，当通过匀浆器获得MMF时，聚合酶活性在60分钟内增加；当通过脱氧胆酸钠裂解制备MMF时，聚合酶活性在120分钟内增加。随后，在37℃孵育150 - 180分钟之间RNA聚合酶活性下降，然后在300分钟内形成平台期。所使用的无细胞系统能够在抗病毒筛选程序中提供有关RNA聚合酶抑制剂测试的有价值信息。
