Anesti Anna-Maria
BioVex Inc, Woburn, MA, USA.
Methods Mol Biol. 2010;617:347-61. doi: 10.1007/978-1-60327-323-7_26.
RNA interference (RNAi) has become a powerful tool for modulating gene expression. While delivery of small interfering RNAs (siRNAs) has achieved silencing of pain-related genes in various animal models of nociception, delivery of short-hairpin RNA (shRNA) or artificial miRNA (miRNA) to dorsal root ganglia (DRG) has proven particularly challenging. This chapter describes a highly efficient method for in vivo gene silencing in sensory neurons using replication-defective vectors based on herpes simplex virus (HSV). This method can be utilised to obtain a better understanding of gene function, validate novel gene targets in drug discovery and potentially develop new RNAi-mediated approaches to achieve analgesia.
RNA干扰（RNAi）已成为调控基因表达的强大工具。虽然小干扰RNA（siRNA）的递送已在各种伤害感受动物模型中实现了与疼痛相关基因的沉默，但将短发夹RNA（shRNA）或人工微小RNA（miRNA）递送至背根神经节（DRG）已证明极具挑战性。本章描述了一种基于单纯疱疹病毒（HSV）的复制缺陷型载体在感觉神经元中进行体内基因沉默的高效方法。该方法可用于更好地理解基因功能、在药物发现中验证新的基因靶点，并有可能开发新的RNAi介导的镇痛方法。
