Department of Anesthesiology and Pain Medicine, Wonkwang University School of Medicine, Iksan, Republic of Korea.
Biofactors. 2010 May-Jun;36(3):210-5. doi: 10.1002/biof.87.
1,2-Dilinoleoyl-sn-glycero-3-phosphocholine (DLPC), the main and active component of soybean lecithin, has been reported to exert anti-inflammatory effects, but the underlying mechanisms remain to be established. It was found that DLPC could induce the expression of the anti-inflammatory heme oxygenase-1 (HO-1) through the activation of nuclear erythroid 2-related factor 2 (Nrf2) in RAW264.7 macrophages. Pretreatment with DLPC suppressed the expression of inducible nitric oxide (NO) synthase (iNOS), one of proinflammatory enzymes, and reduced NO production in lipopolysaccharide (LPS)-stimulated macrophages. Similarly, DLPC also diminished the production of tumor necrosis factor-alpha (TNF-alpha), one of proinflammatory cytokines. Interestingly, the inhibitory effects of DLPC on LPS-induced iNOS expression and TNF-alpha production were reversed by tin protoporphyrin, a HO-1 inhibitor. Thus, HO-1 expression via Nrf2 activation may be one of the possible mechanisms explaining the anti-inflammatory effects of DLPC.
1,2-二亚油酰基-sn-甘油-3-磷酸胆碱（DLPC）是大豆卵磷脂的主要活性成分，据报道具有抗炎作用，但作用机制尚不清楚。研究发现，DLPC 可通过激活 RAW264.7 巨噬细胞中的核红细胞 2 相关因子 2（Nrf2）诱导抗炎血红素加氧酶-1（HO-1）的表达。DLPC 预处理可抑制诱导型一氧化氮合酶（iNOS）的表达，iNOS 是一种促炎酶，可减少脂多糖（LPS）刺激的巨噬细胞中 NO 的产生。同样，DLPC 还可减少促炎细胞因子 TNF-α的产生。有趣的是，HO-1 抑制剂锡原卟啉可逆转 DLPC 对 LPS 诱导的 iNOS 表达和 TNF-α产生的抑制作用。因此，通过 Nrf2 激活 HO-1 的表达可能是解释 DLPC 抗炎作用的机制之一。
