Department of Developmental Biology, section of Genetics, Faculty of Biology, University of Plovdiv, 4000 Plovdiv, Bulgaria.
J Insect Sci. 2008;8:18. doi: 10.1673/031.008.1801.
Isoenzymes are very suitable markers for the study of the inter-breed diversity of the silkworm Bombyx mon L. (Lepidoptera: Bombycidae). More than 250 breeds are raised in Bulgaria, which are not very well studied with regard to their isoenzymic polymorphism. Polymorphism of nonspecific esterases from pupal haemolymph was analyzed, as well as of phosphoglucomutase from different organs of larvae, pupae and imago, from eight introduced breeds. Electrophoresis in polyacrylamide gels was used. A polylocus control of nonspecific esterases, and possible monolocus control of phosphoglucomutase was ascertained. Biallele and triallele polymorphism of phosphoglucomutase locus and in three of the esterase loci was determined. The allelic frequencies of the polymorphic loci in each breed were analyzed. Inter-breed differences were found in different allelic frequencies, different heterozygosity and polymorphism.
同工酶是研究家蚕（鳞翅目：蚕蛾科）种间多样性的非常合适的标记。保加利亚饲养了 250 多个品种，但对其同工酶多态性的研究并不深入。分析了来自 8 个引入品种的幼虫、蛹和成虫不同器官的磷酸葡萄糖变位酶以及蛹血淋巴中非特异性酯酶的多态性。使用聚丙烯酰胺凝胶电泳。确定了非特异性酯酶的多基因控制，以及磷酸葡萄糖变位酶可能的单基因控制。确定了磷酸葡萄糖变位酶基因座的双等位基因和三等位基因多态性，以及 3 个酯酶基因座的三等位基因多态性。分析了每个品种中多态性基因座的等位基因频率。在不同的等位基因频率、不同的杂合度和多态性方面发现了品种间的差异。
