Optical Radiation Standards, National Physical Laboratory, K. S. Krishnan Road, New Delhi-110012, India.
Drug Test Anal. 2009 May;1(5):240-4. doi: 10.1002/dta.41.
5-Fluorouracil is one of the oldest chemotherapy drugs and it has been in use for decades. It is an active medicine against several types of cancer and effectively blocks the replication of DNA viruses. The present study assessed the potential of diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy to determine the thermal stability of 5-fluorouracil. Infrared spectra of the drug before and after exposure to thermal radiation at different temperatures were collected in diffuse reflectance mode using a Fourier transform infrared (FTIR) spectrophotometer. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) analysis were carried out simultaneously to confirm and support the results of infrared spectroscopy. The DRIFT spectra reveal that the drug shows good thermal stability up to 275 degrees C and undergoes complete thermal breakdown at about 285 degrees C. The results of DSC and XRD analysis also give the same information, which support the implementation of diffuse reflectance infrared spectroscopy for the determination of thermal stability of 5-fluorouracil.
5-氟尿嘧啶是最古老的化疗药物之一，已经使用了几十年。它是一种针对多种癌症的有效药物，能有效阻止 DNA 病毒的复制。本研究评估了漫反射红外傅里叶变换（DRIFT）光谱法测定 5-氟尿嘧啶热稳定性的潜力。使用傅里叶变换红外（FTIR）分光光度计以漫反射模式采集药物在不同温度下受热辐射前后的红外光谱。同时进行差示扫描量热法（DSC）和 X 射线衍射（XRD）分析，以确认和支持红外光谱的结果。DRIFT 光谱表明，该药物在 275°C 以下表现出良好的热稳定性，在约 285°C 时完全热分解。DSC 和 XRD 分析的结果也给出了相同的信息，支持采用漫反射红外光谱法测定 5-氟尿嘧啶的热稳定性。
