Sonek J, Gabbe S G, Iams J D, Kniss D A
Department of Obstetrics and Gynecology (Division of Maternal-Fetal Medicine), Ohio State University College of Medicine, Columbus 43210.
Am J Obstet Gynecol. 1991 May;164(5 Pt 1):1174-80. doi: 10.1016/0002-9378(91)90679-l.
Scanning and transmission electron microscopy were used to assess the influence of normal, active labor on the ultrastructure of the human amnion epithelial membrane. Amnion membranes (reflected and placental portions) were obtained from patients either in active labor who were delivered vaginally or by cesarean section after 6 to 12 hours of labor or from patients who underwent elective cesarean section before clinical signs of overt labor. Scanning electron microscopy revealed that reflected amnion membranes that were obtained from patients who were not in labor consisted of a uniform single layer of epithelial cells with numerous microvilli on the apical surface and closely associated cellular borders. In contrast, amnion membranes that were obtained from patients who were in labor consisted of a single layer of epithelial cells, which was interrupted by wide intercellular gaps and extracellular extrusions. Transmission electron microscopy showed that intercellular junctions tended to be less complex in patients who were in labor versus patients who were not in labor. Although lipid droplets were prevalent in both patient groups, specimens that were obtained from patients who were in labor had more lipid droplets per cell than specimens from patients who were not in labor. These results support the theory that the complex biochemical events that culminate in parturition are accompanied and/or preceded by demonstrable morphologic changes in the amnion membrane.
运用扫描电子显微镜和透射电子显微镜评估正常活跃产程对人羊膜上皮膜超微结构的影响。羊膜（反折部和胎盘部）取自经阴道分娩的活跃期产妇，或分娩6至12小时后行剖宫产的产妇，或在未出现明显产程迹象前行择期剖宫产的产妇。扫描电子显微镜显示，取自未临产产妇的反折羊膜由单层均匀上皮细胞构成，其顶端表面有众多微绒毛，细胞边界紧密相连。相比之下，取自临产产妇的羊膜由单层上皮细胞构成，细胞间有宽阔间隙和细胞外突出物，结构被打断。透射电子显微镜显示，与未临产产妇相比，临产产妇的细胞间连接往往没那么复杂。尽管两组患者标本中均有大量脂滴，但取自临产产妇的标本中每个细胞的脂滴比未临产产妇的更多。这些结果支持了这样一种理论，即导致分娩的复杂生化事件伴随着羊膜膜中明显的形态学变化和/或在其之前发生。
