[利用7.0 T磁共振成像通过锰增强磁共振成像追踪大鼠脑皮质脊髓束]
[Manganese enhanced magnetic resonance imaging for tracing corticospinal tracts in rat brain using 7.0 T MRI].
作者信息
Gao Ge-Jun, Wen Song, Yang Tao, Dai Feng, Wu Min, Zang Feng-Chao, An Yan-Li, Tan Zhong-Bao
机构信息
Department of Radiology, Zhongda Hospital, Southeast University, Nanjing 210009, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2010 Jan 26;90(4):269-71.
OBJECTIVE
To investigate the methods of manganese enhanced magnetic resonance imaging in 7.0 T magnetic field for tracing corticospinal tract in rat brain in vivo.
METHODS
0.4 microl volume of 1 mol/L aqueous solution of MnCl(2) was injected into the primary motor cortex of 9 SD rats under stereotaxis. MRI studies were performed for tracing corticospinal tract and other coherent nerve tracts before injection and 24 hours, 48 hours, 72 hours, 7 days after injection respectively using 7.0T Micro-MRI.
RESULTS
Corticospinal tract was visualized perfectly from primary motor cortex, thalamus, cerebral peduncle to pons at different time points after Mn(2+) administration, and the best contrast was achieved after 24-48 h. At the same time, a small quantity of Mn(2+) reached the opposite somatosensory cortex through the corpus callosum.
CONCLUSION
Manganese enhanced MRI visualizes perfectly the transport of Mn(2+) through axoplasmic flow in corticospinal tracts. This method may be used to investigate the change of corticospinal tract and the functional connectivity between two sides of hemisphere in rat brain.
目的
探讨在7.0 T磁场下利用锰增强磁共振成像在体追踪大鼠脑皮质脊髓束的方法。
方法
对9只SD大鼠在立体定位下将0.4微升1 mol/L的MnCl₂水溶液注入初级运动皮层。分别在注射前以及注射后24小时、48小时、72小时、7天利用7.0T微型磁共振成像进行磁共振成像研究以追踪皮质脊髓束及其他相关神经束。
结果
在给予Mn²⁺后的不同时间点，从初级运动皮层、丘脑、大脑脚到脑桥的皮质脊髓束均能清晰显示，在24 - 48小时后对比度最佳。同时，少量Mn²⁺通过胼胝体到达对侧体感皮层。
结论
锰增强磁共振成像能完美显示Mn²⁺通过皮质脊髓束轴浆流的运输情况。该方法可用于研究大鼠脑皮质脊髓束的变化及半球两侧的功能连接。
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