α-促黑素细胞激素在花粉过敏小鼠模型的花粉症发病中起重要作用。
Alpha-melanocyte-stimulating hormone plays an important role in the onset of pollinosis in a pollen allergy mouse model.
机构信息
Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, 1-4-3 Asahimachi, Abeno, Osaka, Japan.
出版信息
Int Arch Allergy Immunol. 2010;153(1):13-8. doi: 10.1159/000301574. Epub 2010 Mar 30.
BACKGROUND
alpha-Melanocyte-stimulating hormone (alpha-MSH) is a neuropeptide that controls melanogenesis in pigmentary cells. In addition, its potent immunomodulatory activity has been recently described in cutaneous inflammatory disorders. However the mechanism of such pollen allergies remains to be elucidated. The purpose of this study was to investigate the role of alpha-MSH in a murine model of pollen allergy.
METHODS
Eight-week-old male BDF-1 mice were sensitized with Cry j I. After the last intranasal antigen, the number of sneezes was counted for 5 min. In addition, the serum levels of IgE and neuronal hormones were measured by ELISA. The expression of IgA, melanocortin receptor 1 (MC1R) and MC5R in the trachea were also observed by immunohistochemistry.
RESULTS
Both the concentration of alpha-MSH and adrenocorticotropin in plasma increase in pollen allergy model mice. Furthermore, MC5R increased in the trachea; however, MC1R did not increase in the trachea. In addition, the changes in sneezing and IgA expression in the pollen allergy model mice were suppressed by alpha-MSH antibody treatment, but they remained unchanged after MC1R antagonist (agouti) treatment.
CONCLUSIONS
These results indicate that sneezing due to pollen allergy is associated with an increased concentration of alpha-MSH and the expression of MC5R.
背景
α-促黑素细胞激素（α-MSH）是一种控制色素细胞中黑色素生成的神经肽。此外，其在皮肤炎症性疾病中的强大免疫调节活性最近也得到了描述。然而，这种花粉过敏的机制仍有待阐明。本研究的目的是研究α-MSH 在花粉过敏的小鼠模型中的作用。
方法
将 8 周龄雄性 BDF-1 小鼠用 Cry j I 致敏。在最后一次鼻腔抗原后，计数 5 分钟内的喷嚏次数。此外，通过 ELISA 测量血清 IgE 和神经激素水平。还通过免疫组织化学观察气管中 IgA、黑素皮质素受体 1（MC1R）和 MC5R 的表达。
结果
花粉过敏模型小鼠血浆中 α-MSH 和促肾上腺皮质激素的浓度均增加。此外，气管中 MC5R 增加；然而，气管中 MC1R 没有增加。此外，α-MSH 抗体治疗可抑制花粉过敏模型小鼠的打喷嚏和 IgA 表达变化，但 MC1R 拮抗剂（阿黑皮素原）治疗后无变化。
结论
这些结果表明，花粉过敏引起的打喷嚏与 α-MSH 浓度增加和 MC5R 表达有关。
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