Kim Sun-Il, Pham Thi Tra, Lee Jae-Wook, Roh Sung-Hee
Department of Chemical and Biochemical Engineering, Chosun University, Gwangju 501-759, Korea.
J Nanosci Nanotechnol. 2010 May;10(5):3425-9. doi: 10.1166/jnn.2010.2323.
Mesoporous silica spheres (SBA-15) have been obtained via a two-step synthesis process by using a triblock copolymer as a template in combination with a co-surfactant and co-solvent. Multi-amine-grafted mesoporous silicas were prepared by attaching 3-aminopropyl triethoxysilane, N-2(-aminoethyl)-3-aminopropyltrimethoxysilane and (3-trimethoxysilylpropyl)diethylenetriamine via a post-synthetic method. The proteins used in adsorption experiments include Bovine Serum Albumin (BSA), Lysozyme (LYS) and Myoglobin (MYO). The protein adsorption properties such as equilibrium and kinetics were investigated. The results show that the original SBA-15 samples have the highest adsorption capacity for all proteins due to their largest pore size and internal surface area.
介孔二氧化硅球（SBA - 15）是通过两步合成工艺制备的，该工艺使用三嵌段共聚物作为模板，并结合共表面活性剂和共溶剂。通过后合成方法连接3 - 氨丙基三乙氧基硅烷、N - 2（-氨乙基）- 3 - 氨丙基三甲氧基硅烷和（3 - 三甲氧基硅丙基）二乙烯三胺，制备了多胺接枝介孔二氧化硅。吸附实验中使用的蛋白质包括牛血清白蛋白（BSA）、溶菌酶（LYS）和肌红蛋白（MYO）。研究了蛋白质吸附的平衡和动力学等性质。结果表明，原始的SBA - 15样品由于其最大的孔径和内表面积，对所有蛋白质具有最高的吸附容量。
