载脂蛋白 M 基因近端启动子区单核苷酸多态性与冠状动脉疾病的关系。
Determination of single-nucleotide polymorphism in the proximal promoter region of apolipoprotein M gene in coronary artery diseases.
机构信息
Comprehensive Laboratory, The Third Affiliated Hospital, Suzhou University, Changzhou China;
出版信息
Int J Gen Med. 2009 Dec 29;2:177-82. doi: 10.2147/ijgm.s4687.
OBJECTIVE
It has been reported that single-nucleotide polymorphism (SNP) in the proximal promoter region of apolipoprotein M (apoM) gene may confer the risk in the development of type 2 diabetes (T2D) and coronary artery disease (CAD) in the Han Chinese. However, in a recent study demonstrated that plasma apoM level did not correlated to the coronary heart disease. In the present studies, we investigated the SNP T-778C of apoM gene in CAD patients and controls in the Han Chinese population. Moreover we examined whether serum apoM levels could be influenced by this promoter mutation.
MATERIAL AND METHODS
One hundred twenty-six CAD patients and 118 non-CAD patients were subjected in the present study. All patients were confirmed by the angiography. The genotyping of polymorphisms T-778C in apoM promoter was determined by real-time polymerase chain reaction. Serum apoM levels were semi-quantitatively determined by the dot-blotting analysis.
RESULTS
Distribution of apoM T-778C genotype in non-CAD patients was as following: 84.7% were T/T, 15.3% were T/C and 0.0% was C/C. T allele frequencies were 92.4% and C allele, 7.6%. In the CAD patients, 99 patients (78.6%) had the T/T genotype, 25 patients (19.8%) with T/C genotype and 2 patients (1.6%) with C/C genotype. The allele frequency was 88.5% for the T allele and 11.5% for the C allele. There was no statistical significant difference of serum apoM levels found in these three genotypes.
CONCLUSIONS
There was no significant difference in allele or genotype frequencies between CAD patients and non-CAD patients. Binary logistic regression analysis with adjustments for age, gender, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, apoAI, apoB, and LP(a) indicated that the TC and CC genotypes in SNP T-778C were not significantly associated with the development of CAD (odds ratio = 1.510, 95% confidence interval: 0.756-3.017; p = 0.243).
目的
据报道，载脂蛋白 M（apoM）基因近端启动子区域的单核苷酸多态性（SNP）可能使汉族人易患 2 型糖尿病（T2D）和冠心病（CAD）。然而，最近的一项研究表明，血浆 apoM 水平与冠心病无关。在本研究中，我们调查了汉族人群 CAD 患者和对照组中 apoM 基因 SNP T-778C。此外，我们还研究了该启动子突变是否会影响血清 apoM 水平。
材料与方法
本研究纳入 126 例 CAD 患者和 118 例非 CAD 患者。所有患者均经血管造影证实。采用实时聚合酶链反应检测 apoM 启动子多态性 T-778C 的基因分型。采用斑点印迹法半定量检测血清 apoM 水平。
结果
非 CAD 患者 apoM T-778C 基因型分布如下：84.7%为 T/T，15.3%为 T/C，0.0%为 C/C。T 等位基因频率为 92.4%，C 等位基因频率为 7.6%。在 CAD 患者中，99 例（78.6%）为 T/T 基因型，25 例（19.8%）为 T/C 基因型，2 例（1.6%）为 C/C 基因型。T 等位基因频率为 88.5%，C 等位基因频率为 11.5%。在这三种基因型中，血清 apoM 水平无统计学差异。
结论
CAD 患者与非 CAD 患者之间等位基因或基因型频率无显著差异。经年龄、性别、甘油三酯、总胆固醇、低密度脂蛋白、高密度脂蛋白、载脂蛋白 AI、载脂蛋白 B 和脂蛋白（a）校正的二元逻辑回归分析表明，SNP T-778C 的 TC 和 CC 基因型与 CAD 的发生无显著相关性（比值比=1.510，95%置信区间：0.756-3.017；p=0.243）。
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