基于金纳米粒子增强作用的无标记及裸眼检测 PNA/DNA 杂交
Label-free and naked eye detection of PNA/DNA hybridization using enhancement of gold nanoparticles.
机构信息
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Republic of Korea.
出版信息
Chem Commun (Camb). 2010 May 21;46(19):3315-7. doi: 10.1039/b926940a. Epub 2010 Apr 1.
DOI:10.1039/b926940a
PMID:20361103
Abstract
Utilizing a gold enhancement process after inducing electrostatic interaction between positively charged gold nanoparticles and negatively charged target DNA hybridized to neutral PNA capture probes, a new method for label-free detection of DNA was developed and successfully applied to detect H5-type DNA.
摘要
利用金增强过程，在带正电荷的金纳米粒子与杂交到中性 PNA 捕获探针上的带负电荷的靶 DNA 之间诱导静电相互作用后，开发了一种用于无标记检测 DNA 的新方法，并成功应用于检测 H5 型 DNA。
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