Abbott RealTime HCV Genotype II assay 的多中心评估。
A multicenter evaluation of the Abbott RealTime HCV Genotype II assay.
机构信息
Laboratory of Molecular Virology, Foundation University Hospital Tor Vergata, Viale Oxford, 81-00133, Rome, Italy.
出版信息
J Virol Methods. 2010 Aug;167(2):205-7. doi: 10.1016/j.jviromet.2010.03.017. Epub 2010 Mar 31.
Genotype determination is recommended before starting anti-HCV therapy to determine the duration of treatment (PEG-Interferon+ribavirin). The Versant HCV Genotype 2.0 assay, based on the reverse hybridization of the 5'UTR segment and core region of hepatitis C virus (HCV), has been one of the assays used most widely for HCV genotyping. A multicenter evaluation of the more automated Abbott RealTime HCV Genotype II assay was carried out on 124 HCV positive sera tested previously with the Versant HCV Genotype 2.0 assay. There was good agreement between the two assays. Type concordance was 95.9% (117/122) while concordance at the subtype level for genotype 1 was 95.6% (43/45). The Abbott RealTime HCV Genotype II assay is automated, allowing a substantial reduction of time-to results and hands-on time. The combined features of full automation, objective interpretation and digital archiving make this assay useful in a diagnostic setting.
建议在开始抗 HCV 治疗之前进行基因型测定，以确定治疗时间（PEG-干扰素+利巴韦林）。基于丙型肝炎病毒（HCV）5'UTR 片段和核心区域的反向杂交的 Versant HCV Genotype 2.0 检测法是最广泛用于 HCV 基因分型的检测法之一。对之前使用 Versant HCV Genotype 2.0 检测法检测的 124 份 HCV 阳性血清进行了更自动化的 Abbott RealTime HCV Genotype II 检测法的多中心评估。两种检测法之间具有良好的一致性。类型一致性为 95.9%（117/122），而基因型 1 的亚型水平一致性为 95.6%（43/45）。Abbott RealTime HCV Genotype II 检测法是自动化的，可大大减少获得结果的时间和人工操作时间。完全自动化、客观解释和数字存档的综合功能使该检测法在诊断环境中非常有用。
Zotero 插件