[生存素、半胱天冬酶-3在口腔癌前病变及口腔鳞状细胞癌中的表达]

[Expression of survivin, caspase-3 in oral precancerous lesions and oral squamous-cell carcinoma].

作者信息

Ding Yan-ping, Li Shu-xia, Wu Hong-ru, Zhang Xin-yan, Tang Xiao-fei, Sun Zheng

机构信息

Department of Oral Medicine, Capital Medical University School of Stomatology, Beijing 100051, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2010 Feb;45(2):85-8.

PMID:20368001

Abstract

OBJECTIVE

To investigate the roles of surviving and caspase-3 in the development of oral cancer.

METHODS

Archival tissue sections of 17 oral squamous cell carcinoma (OSCC), 28 oral leukoplakia with dysplasia, 10 normal oral mucosa were obtained from Capital Medical University School of Stomatology for immunohistochemical staining of markers of survivin and caspase-3. The cell apoptosis was detected with terminal deoxynucleotidyl transferase-mediated nucleotide shift enzyme (TdT) mediated d-UTP end labeling (TUNEL). Positively stained cells were counted and analyzed statistically to determine potential relationship between survivin, caspase-3 and cell apoptosis.

RESULTS

The expression of survivin was faint or negative in normal epithelial cells. The average positive rate of survivin was (1.05 ± 1.21)% in control group and (21.89 ± 10.45)% in OSCC. Caspase-3 was expressed in all the normal mucosa,but it obviously down-regulated in dysplasia and OSCC. The apoptosis index (AI) decreased from (0.89 ± 0.46)% in normal mucosa to (0.21 ± 0.12)% in OSCC.

CONCLUSIONS

Both survivin and caspase-3 are associated with carcinogenesis of the oral mucosa. Survivin may restrain cell apoptosis by inhibiting caspase-3.

摘要

目的

探讨生存素（Survivin）和半胱天冬酶-3（Caspase-3）在口腔癌发生发展中的作用。

方法

从首都医科大学口腔医学院获取17例口腔鳞状细胞癌（OSCC）、28例发育异常的口腔白斑及10例正常口腔黏膜的存档组织切片，进行生存素和Caspase-3标志物的免疫组化染色。采用末端脱氧核苷酸转移酶介导的缺口末端标记法（TUNEL）检测细胞凋亡情况。对阳性染色细胞进行计数并统计学分析，以确定Survivin、Caspase-3与细胞凋亡之间的潜在关系。

结果

Survivin在正常上皮细胞中表达微弱或呈阴性。对照组Survivin平均阳性率为（1.05±1.21）%，OSCC组为（21.89±10.45）%。Caspase-3在所有正常黏膜中均有表达，但在发育异常及OSCC中明显下调。凋亡指数（AI）从正常黏膜的（0.89±0.46）%降至OSCC的（0.21±0.12）%。