通过电穿孔法转染小鼠耳蜗外植体。
Transfection of mouse cochlear explants by electroporation.
作者信息
Driver Elizabeth C, Kelley Matthew W
机构信息
National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland, USA.
出版信息
Curr Protoc Neurosci. 2010 Apr;Chapter 4:Unit 4.34.1-10. doi: 10.1002/0471142301.ns0434s51.
Abstract
The sensory epithelium of the mammalian inner ear, also referred to as the organ of Corti, is a remarkable structure comprised of highly ordered rows of mechanosensory hair cells and non-sensory supporting cells located within the coiled cochlea. This unit describes an in vitro explant culture technique that can be coupled with gene transfer via electroporation to study the effects of altering gene expression during development of the organ of Corti. While the protocol is largely focused on embryonic cochlea, the same basic protocol can be used on cochleae from mice as old as P5.
摘要
哺乳动物内耳的感觉上皮，也称为柯蒂氏器，是一种非凡的结构，由位于螺旋状耳蜗内的高度有序排列的机械感觉毛细胞和非感觉支持细胞组成。本单元介绍了一种体外外植体培养技术，该技术可与通过电穿孔进行的基因转移相结合，以研究在柯蒂氏器发育过程中改变基因表达的影响。虽然该方案主要针对胚胎耳蜗，但相同的基本方案可用于P5龄小鼠的耳蜗。
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