Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, Denmark.
J Innate Immun. 2010;2(2):167-80. doi: 10.1159/000218324. Epub 2009 May 8.
The three human ficolins, H-ficolin, L-ficolin and M-ficolin, are pattern recognition molecules of the innate immune system. All three ficolins can activate the lectin pathway of the complement system after binding to pathogens. H- and L-ficolin are serum proteins with an average concentration of 18 and 3 microg/ml, respectively. M-ficolin has been described as a membrane-associated pattern recognition receptor of monocytes, being also present in granulocytes; recently, minuscule amounts of M-ficolin have been found in serum, too. No assay specific for M-ficolin has yet been described and biological variations are unknown. We have raised specific monoclonal anti-human M-ficolin antibodies and have developed a quantitative assay for M-ficolin. M-ficolin elutes as a large, 900-kDa protein upon gel permeation chromatography of serum. Analysis of M-ficolin levels in serum samples of 350 blood donors reveals a mean concentration of 1.07 microg/ml, ranging from 0.28 to 4.05 microg/ml. Analyses of consecutive acute phase serum samples from major surgery patients indicated a complex response. Ontogeny was investigated through cord blood samples from healthy full-term babies, which showed adult levels, with sequential samples showing no increase from birth to 1 year of age. We suggest that M-ficolin should also be considered as a humoral pattern recognition molecule.
三种人类ficolins（H 型 ficolin、L 型 ficolin 和 M 型 ficolin）是先天免疫系统的模式识别分子。三种 ficolin 结合病原体后都能激活补体系统的凝集素途径。H 型 ficolin 和 L 型 ficolin 是血清蛋白，平均浓度分别为 18 和 3μg/ml。M 型 ficolin 被描述为单核细胞的膜结合模式识别受体，也存在于粒细胞中；最近，在血清中也发现了少量的 M 型 ficolin。目前尚未描述专门用于 M 型 ficolin 的检测方法，其生物学变化也未知。我们已经制备了特异性的单克隆抗人 M 型 ficolin 抗体，并开发了一种 M 型 ficolin 的定量检测方法。M 型 ficolin 在血清的凝胶过滤层析中洗脱为一个 900kDa 的大分子。对 350 名献血者血清样本中 M-ficolin 水平的分析显示，其平均浓度为 1.07μg/ml，范围为 0.28-4.05μg/ml。对大手术患者连续急性期血清样本的分析表明，存在复杂的反应。通过对健康足月婴儿脐带血样本的研究，发现其成人生理水平，连续样本显示从出生到 1 岁没有增加。我们认为 M 型 ficolin 也应被视为一种体液模式识别分子。
Zotero 插件