Reeves W K, Peiris K H S, Scholte E-J, Wirtz R A, Dowell F E
U.S. Department of Agriculture, Agricultural Research Services (USDA ARS), Arthropod-Borne Animal Diseases Research Laboratory, Laramie, WY, USA.
Med Vet Entomol. 2010 Mar;24(1):32-7. doi: 10.1111/j.1365-2915.2009.00843.x.
Age-grading of insects is important in the control and monitoring of both insect populations and vector-borne diseases. Microscopy and morphological techniques exist to age-grade most blood-feeding flies, but these techniques are laborious, often destructive to the insects, and slow. Near-infrared spectroscopy (NIRS) can be automated and is a non-destructive technique for age-grading. We applied NIRS techniques to age-grade females of the biting midge, Culicoides sonorensis Wirth & Jones (Diptera: Ceratopogonidae), the vector of bluetongue and other arboviruses in North America. Female flies of five known age cohorts (1, 3, 6, 9 and 12 days post-emergence) from three laboratory colonies were used. The data indicate that NIRS can be used to differentiate age groups of C. sonorensis.
昆虫的年龄分级对于昆虫种群控制和媒介传播疾病监测都很重要。现有显微镜检查和形态学技术可用于对大多数吸血蝇进行年龄分级，但这些技术费力、往往对昆虫具有破坏性且速度慢。近红外光谱（NIRS）可以实现自动化，是一种用于年龄分级的非破坏性技术。我们应用NIRS技术对北美蓝舌病及其他虫媒病毒的传播媒介——刺吸蠓（Culicoides sonorensis Wirth & Jones，双翅目：蠓科）的雌虫进行年龄分级。使用了来自三个实验室种群的五个已知年龄组（羽化后1、3、6、9和12天）的雌蝇。数据表明，NIRS可用于区分索诺拉库蠓的年龄组。
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