系统性抗肿瘤坏死因子-α治疗对 2 型糖尿病小鼠牙龈卟啉单胞菌感染的影响。
The effect of systemic anti-tumor necrosis factor-alpha treatment on Porphyromonas gingivalis infection in type 2 diabetic mice.
机构信息
Nihon University Graduate School of Dentistry, Tokyo, Japan.
出版信息
Arch Oral Biol. 2010 May;55(5):379-84. doi: 10.1016/j.archoralbio.2010.03.004. Epub 2010 Apr 8.
OBJECTIVE
Diabetes mellitus and periodontal disease are two common chronic diseases that have long been thought to be biologically linked. Overexpression of tumor necrosis factor alpha (TNF-alpha) is thought to contribute to this bidirectional inter-relationship. This study examined the effect of anti-TNF-alpha antibody treatment on Porphyromonas gingivalis infection in diabetic mice.
METHODS
In C57BL/6 (normal) and KKAy (diabetic) mice, the area adjacent to the periosteum at a point on the skull midway between the ears was inoculated with P. gingivalis. At 24h after the inoculation, the mice in the test group were treated with rat anti-murine TNF-alpha intravenously, while the control group received non-immunized rat IgG. TNF-alpha, IL-6, and fasting blood glucose levels in the mice were measured on day 3.
RESULTS
Anti-TNF-alpha antibody treatment improved the host response to P. gingivalis and was associated with reduced serum TNF-alpha, IL-6, and fasting blood glucose levels in the KKAy mice. Anti-TNF-alpha antibody treatment also decreased the lesion size at the P. gingivalis inoculation.
CONCLUSIONS
Our results suggest that TNF-alpha plays a role in the two-way relationship between P. gingivalis infection and diabetes mellitus. Anti-TNF-alpha antibody treatment may improve the host response to P. gingivalis infection and glycemic control in diabetes mellitus.
目的
糖尿病和牙周病是两种常见的慢性疾病，长期以来一直被认为存在生物学联系。肿瘤坏死因子-α（TNF-α）的过度表达被认为促成了这种双向的相互关系。本研究探讨了抗 TNF-α 抗体治疗对糖尿病小鼠牙龈卟啉单胞菌感染的影响。
方法
在 C57BL/6（正常）和 KKAy（糖尿病）小鼠中，在耳间中点颅骨骨膜附近的一个点接种牙龈卟啉单胞菌。接种后 24 小时，实验组小鼠经静脉注射抗鼠 TNF-α 抗体，对照组给予非免疫性大鼠 IgG。第 3 天测量小鼠的 TNF-α、IL-6 和空腹血糖水平。
结果
抗 TNF-α 抗体治疗改善了宿主对牙龈卟啉单胞菌的反应，与 KKAy 小鼠血清 TNF-α、IL-6 和空腹血糖水平降低有关。抗 TNF-α 抗体治疗还降低了牙龈卟啉单胞菌接种部位的病变大小。
结论
我们的结果表明 TNF-α 在牙龈卟啉单胞菌感染与糖尿病之间的双向关系中起作用。抗 TNF-α 抗体治疗可能改善宿主对牙龈卟啉单胞菌感染的反应和糖尿病的血糖控制。
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